Rapid Diagnostic Testing of Urinary Tract Infections

尿路感染的快速诊断检测

基本信息

批准号：
8014607
负责人：
Ronald J Rieder
金额：
$ 2.61万
依托单位：
BIOSENSE TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-05 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8014607
关键词：
Accounting Adult Algorithms Amoxicillin Antibiotic Resistance Antibiotic susceptibility Antibiotics Antimicrobial Resistance Applications Grants Area Attention Bacteria Bacterial Infections Biological Biosensing Techniques Businesses Ceftriaxone Cell Count Cell division Cells Chemicals Child Ciprofloxacin Clavulanate Clinic Clinical Clinical Microbiology Collection Country Detection Development Devices Diagnostic Diagnostic Procedure Diagnostic tests Dimensions Electrodes Electronics Elements Enterococcus faecium Escherichia coli Event Exposure to FDA approved Figs - dietary Fluoroquinolones Funding Generations Genus staphylococcus Grant Growth Healthcare Hospitals Hour Housing Infection Investigation Kanamycin Klebsiella pneumonia bacterium Laboratories Levaquin Life Measurement Measures Medical center Metabolic Metabolic Pathway Methods Mirabilis Monitor National Institute of Diabetes and Digestive and Kidney Diseases Nitrofurantoin Norfloxacin Optics Organism Outcome Outpatients Patients Performance Pharmaceutical Preparations Phase Physiological Population Predisposition Procedures Process Production Proteus mirabilis Protocols documentation Pseudomonas aeruginosa Records Research Resistance Serratia marcescens Shock Side Specimen Speed Staging Staphylococcus aureus Streptococcus Streptococcus Group B Stress Suspension substance Suspensions Techniques Technology Testing Time Trimethoprim-Sulfamethoxazole United States Urinary tract infection Urine Uropathogen Visit Woman Work abstracting antimicrobial antimicrobial drug authority bacterial resistance base biological adaptation to stress cost cost effective data acquisition design and construction effectiveness measure electric impedance improved innovation instrument instrumentation millimeter novel operation pathogen pathogenic bacteria phase 1 study phase 2 study prevent programs prototype research study resistant strain sample collection stressor tool urinary

项目摘要

DESCRIPTION (provided by applicant): Abstract The continued development of a rapid diagnostic method for testing the antibiotic susceptibility of urinary tract infections in near real-time is proposed. Currently, all methods for determining antibiotic susceptibility rely exclusively on detecting changes in the bacterial population caused by cell division after exposure to an antimicrobial compound. In our Phase I effort we demonstrated a novel phenotypic approach to antibiotic susceptibility testing that provides commensurate information without measurements of growth. Technical feasibility of this method was demonstrated by measuring the antibiotic susceptibility of both sensitive and resistant strains of Escherichia coli and Pseudomonas aeruginosa treated with antibiotics having different mechanisms of action. Susceptibility results were obtained within one hour time. In addition, resistant strains were easily distinguished from sensitive strains. Because grown cultures are no longer needed to obtain results, susceptibility results can be obtained directly from urinary specimens at the point of collection within 90 minutes total reducing the time from sample collection to availability by days enabling targeted therapy, improved patient outcomes, and the reduced spread and generation of resistant organisms. The method can be realized with a disposable device that is simple, cost effective, and ideal for mass production. The proposed Phase II effort focuses on extending our investigation to include all first and second line antibiotics used to treat uropathogens and developing a prototype test instrument. Project Narrative Urinary tract infections (UTIs) are among the most common of all bacterial infections, occurring primarily in women, and accounting for as many as 8 million outpatient visits to doctors annually. Furthermore, UTIs have become the leading healthcare-associated infection among adults and children in the United States. However, the management of UTIs has become more complicated over the last decade due to increasing antimicrobial resistance to first-line antibiotics. Already, authorities advise the use of significantly more expensive fluoroquinolones as a first line treatment for uncomplicated UTIs when the prevailing rate of antibiotic resistance in the area rises to greater than 20 percent. The same type of resistance of E. coli in other countries has reached a shocking 70 to 100 percent. Antibiotic resistance represents a problem of increasing societal importance brought about by the overuse or inappropriate use of broad-spectrum antibiotics. The proposed rapid test is an essential tool for the responsible and prudent use of prescribed antibiotics to prevent the spread of resistant bacterial strains.

描述（由申请人提供）：抽象的建议继续开发一种快速诊断方法，用于近实时测试尿路感染的抗生素敏感性。目前，所有确定抗生素敏感性的方法都完全依赖于检测暴露于抗菌化合物后由细胞分裂引起的细菌群体的变化。在我们的第一阶段工作中，我们展示了一种新颖的抗生素敏感性测试表型方法，该方法可以提供相应的信息，而无需测量生长。通过测量用不同作用机制的抗生素处理的大肠杆菌和铜绿假单胞菌的敏感菌株和耐药菌株的抗生素敏感性，证明了该方法的技术可行性。一小时内获得药敏结果。此外，抗性菌株与敏感菌株很容易区分。由于不再需要生长的培养物来获得结果，因此可以在 90 分钟内从收集点的尿液样本中直接获得药敏结果，从而将样本收集到可用的时间缩短了数天，从而实现靶向治疗、改善患者结果并减少耐药生物体的传播和产生。该方法可以使用一次性装置来实现，该装置简单、成本有效并且非常适合大规模生产。拟议的第二阶段工作重点是扩大我们的研究范围，以包括用于治疗尿路病原体的所有一线和二线抗生素，并开发原型测试仪器。项目叙述尿路感染 (UTI) 是所有细菌感染中最常见的一种，主要发生于女性，每年门诊就诊人数多达 800 万人次。此外，尿路感染已成为美国成人和儿童中主要的医疗保健相关感染。然而，在过去十年中，由于一线抗生素的耐药性不断增加，尿路感染的治疗变得更加复杂。当局已经建议，当该地区普遍存在的抗生素耐药率上升至 20% 以上时，使用价格昂贵得多的氟喹诺酮类药物作为无并发症尿路感染的一线治疗方法。其他国家的同类型大肠杆菌耐药率已达到令人震惊的70%至100%。抗生素耐药性是由于过度使用或不当使用广谱抗生素而引起的日益重要的社会问题。拟议的快速检测是负责任和谨慎使用处方抗生素以防止耐药菌株传播的重要工具。