Rapid Detection of Bacterial Contamination in Platelets

快速检测血小板中的细菌污染

• 批准号: 6879291
• 负责人: Ronald J Rieder
• 金额: $ 4.86万
• 依托单位: BIOSENSE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2006-08-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879291
• 关键词: Escherichia coli; Serratia marcescens; Staphylococcus aureus; Staphylococcus epidermidis; bacterial disease; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; biosensor device; biotechnology; bioterrorism /chemical warfare; blood bank /supply contamination; communicable disease diagnosis; diagnosis design /evaluation; electrical impedance; human tissue; microorganism growth; platelet transfusion; platelets; rapid diagnosis; transplantation disease transmission

DESCRIPTION (provided by applicant): The development of a rapid and simple method for the diagnostic testing of pathogenic bacteria in platelet products is proposed. The technical approach is based on a novel implementation of impedance sensing to monitor cellular growth with unprecedented sensitivity and stability. This enables results to be obtained directly from platelet samples within hours with a simple, inexpensive, and easy to use device ideally suited for mass production. The innovative method avoids the need for high cell densities, thus reducing the time from sample collection to detection of bacterial contamination from days to hours. Feasibility of the diagnostic tool will be demonstrated with several of the clinically significant bacterial strains which are often implicated in transfusion-related sepsis.

描述（由申请人提供）：建议开发一种快速且简单的方法来诊断检测血小板产品中的病原菌。该技术方法基于阻抗传感的新颖实现，以前所未有的灵敏度和稳定性监测细胞生长。这使得能够在数小时内通过简单、廉价且易于使用的设备直接从血小板样本中获得结果，非常适合大规模生产。这种创新方法避免了对高细胞密度的需求，从而将从样品采集到检测细菌污染的时间从几天缩短到几小时。该诊断工具的可行性将通过几种临床上重要的细菌菌株来证明，这些菌株通常与输血相关的败血症有关。