Diagnosis of Extra-Pulmonary Tuberculosis in HIV Patients

HIV患者肺外结核的诊断

基本信息

批准号：
7417417
负责人：
Ronald J Rieder
金额：
$ 10万
依托单位：
BIOSENSE TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-15 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7417417
关键词：
Acquired Immunodeficiency Syndrome Address Antibiotic susceptibility Applications Grants Bacteria Behavior Biological Assay Biosensing Techniques Biosensor Blood Blood Cells Blood Platelets Blood specimen Businesses Cell Count Cell Survival Cells Centrifugation Chemicals Clinical Communicable Diseases Compatible Condition Culture Media Cytolysis Detection Developed Countries Developing Countries Development Devices Diagnosis Diagnostic Diagnostic tests Dimensions Disease Disease Progression Doctor of Medicine Electrodes Elements Environment Erythrocytes Event Exposure to General Population Genus Mycobacterium Goals Growth HIV Housing Human Individual Investigation Laboratories Left Letters Leukocytes Life Lung Massachusetts Measurement Measures Metabolic Metabolic stress Methods Microscopy Monitor Mycobacterium smegmatis Mycobacterium tuberculosis Operative Surgical Procedures Organism Patients Pharmaceutical Preparations Phase Plasma Predisposition Preparation Prevalence Process Production Protocols documentation Pulmonary Tuberculosis Rate Reading Records Research Sampling Savings Screening procedure Shock Solid Specimen Speed Sputum Staging Standards of Weights and Measures Stress Suspension substance Suspensions Techniques Technology Testing Time Tuberculosis Virus Week Whole Blood Work base biological adaptation to stress cost cost effective design dielectric property disorder control electric impedance immunosuppressed improved innovation instrumentation interest laptop medical schools microorganism novel professor programs prototype rapid detection rapid diagnosis rapid technique research clinical testing research study response sample collection sensor size stressor thermal stress tool

项目摘要

DESCRIPTION (provided by applicant): The diagnosis of tuberculosis (TB) in individuals co-infected with human immunodeficiency virus is significantly challenged by the increased prevalence of extra-pulmonary forms of TB in severely immuno- suppressed patients. A cost-effective method for the rapid screening of mycobacteria in blood from HIV infected patients is proposed. The technical approach relies on monitoring the metabolic behavior of mycobacteria with exceptional sensitivity and stability using impedance sensing methods. Corresponding induced metabolic responses allow the rapid detection of viable slow-growing organisms in near-real time. This enables diagnostic results to be obtained without the need for grown cultures reducing the time from sample collection to complete diagnosis by many weeks compared to currently used methods. Feasibility of the proposed diagnostic tool will be demonstrated with strains of Mycobacterium smegmatis and Mycobacterium tuberculosis within a 6-month Phase I effort. A Phase II effort will be focused on the development of a prototype instrumentation and clinical testing with M. tuberculosis samples.Project Narrative A rapid diagnostic test will be developed for detecting the slow-growing bacteria responsible for the disease tuberculosis in severely immunosuppressed individuals co-infected with HIV. The proposed test offers a significant increase in speed over solid media growth and a significant gain in sensitivity over smear microscopy ideal for screening use in developing countries. This enables fewer false negative diagnoses and the positive diagnosis of TB earlier in the disease progression. The impact of this is the improved ability to control the disease and fewer untreated patients returning to the general population to infect others and spread the disease.

描述（由申请人提供）：由于严重免疫抑制患者中肺外结核病患病率的增加，人类免疫缺陷病毒合并感染个体的结核病（TB）诊断受到重大挑战。提出了一种快速筛查 HIV 感染患者血液中分枝杆菌的经济有效的方法。该技术方法依赖于使用阻抗传感方法以卓越的灵敏度和稳定性监测分枝杆菌的代谢行为。相应的诱导代谢反应可以近乎实时地快速检测存活的缓慢生长的生物体。这使得无需培养培养物即可获得诊断结果，与目前使用的方法相比，从样本采集到完成诊断的时间缩短了数周。所提出的诊断工具的可行性将在为期 6 个月的第一阶段工作中通过耻垢分枝杆菌和结核分枝杆菌菌株进行验证。第二阶段的工作将集中于原型仪器的开发和结核分枝杆菌样本的临床测试。项目叙述将开发一种快速诊断测试，用于检测在严重免疫抑制的同时感染艾滋病毒的个体中导致结核病的缓慢生长的细菌。所提出的测试显着提高了固体培养基的生长速度，并且显着提高了涂片显微镜的灵敏度，非常适合发展中国家的筛查使用。这样可以减少假阴性诊断，并在疾病进展的早期对结核病进行阳性诊断。其影响是提高了控制疾病的能力，并且减少了未经治疗的患者返回普通人群感染他人和传播疾病的情况。