LSM 710 Zeiss Confocal Microscope

LSM 710 蔡司共焦显微镜

• 批准号: 7795000
• 负责人: JUDITH K CHRISTMAN
• 金额: $ 46.11万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795000
• 关键词: Arts; Cells; Confocal Microscopy; Core Facility; Detection; Disease; Equipment; Floor; Funding; Goals; Grant; Housing; Laboratories; Lasers; Life; Malignant Neoplasms; Medicine; Microscope; Minor; Recruitment Activity; Research; Research Institute; Research Personnel; Research Project Grants; Scientist; Services; UNMC Eppley Cancer Center; United States National Institutes of Health; college; fluorophore; instrument; meetings; programs; protein protein interaction; protein transport

DESCRIPTION (provided by applicant): The Confocal Microscopy Core Facility (CMCF) is housed in the Durham Research Center (DRC), which opened in 2006. The CMCF was initiated at UNMC in 1996 and has served the research goals of >80 scientists since then. It is used extensively by scientists from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases and the UNMC/ Eppley Cancer Center. The CMCF now maintains two confocal microscopes, a Zeiss LSM 410 and a Zeiss LSM 510 Meta. In 2006, the SIG program funded the purchase of an LSM 510 which was upgraded to an LSM 510Ma with funds provided by Vice Chancellor for Research at UNMC. The LSM 510M was put into service in early 2007, shortly after the CMCF moved to the DRC. This 10 story building, containing 136 laboratories will now become DRC I; an adjacent tower (DRC II) containing an additional 98 laboratories with added core facility space on the first floor will open in May. With few exceptions, occupancy of the lab modules is limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging > $200,000/year/module. This means that the CMCF can realistically expect an increase in the number of NIH funded investigators with need to access the CMCF for their NIH funded research. In 2006, we planned to keep both the LSM 410 and 510M in use to cover the needs of an increased number of investigators. However, only a few of our users are willing to use the limited capacity of this instrument. In contrast, the LSM 510M is in almost constant use. A technician was added to the staff so that we now have two knowledgeable people to provide assistance to investigators on weekdays from 7:30 AM to 7:30 PM (or later in special situations). Before grant dead-lines and meetings, there is often a 4-5 day wait to access the LSM 510M. Thus, it is imperative to increase our capacity. Our objective with this request for an LSM 710 is to continue providing state of the art facilities and to expand the capacity of our core to accommodate an increasing pool of NIH users. Confocal usage (hrs) in the CMCF has almost doubled since 2005 and continues to grow, making the purchase of the LSM 710 a critical need. The requested LSM 710 with Quasar spectral detection, 34 spectral detection channels and 6 laser lines- 405, 458, 488, 514, 561 and 633 nm and incubation equipment will allow the users to perform optimized co- localization of multiple fluorophores and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects detailed in this proposal are selected from those of 11 major users and 23 minor users.

描述（由申请人提供）：共聚焦显微镜核心设施（CMCF）位于达勒姆研究中心（DRC），该中心于2006年开业。CMCF于1996年在UNMC启动，此后一直为80位科学家提供了> 80个科学家的目标。它由医学院，埃普利癌症和相关疾病研究所以及UNMC/ Eppley癌症中心的科学家广泛使用。 CMCF现在维持两个共聚焦显微镜，一个Zeiss LSM 410和一个Zeiss LSM 510 meta。 2006年，SIG计划资助了购买LSM 510的计划，该510升级到LSM 510MA，并由副校长提供的资金在UNMC进行。 CMCF搬到刚果民主共和国后不久，LSM 510m于2007年初投入使用。这10个故事建设，其中包含136个实验室，现在将成为DRC I；一个相邻的塔楼（DRC II），其中包含额外的98个实验室，并将在5月在一楼增加核心设施空间。除少数例外，实验室模块的占用限制仅限于当前和新招募的研究人员，其积极的研究补助金（NIH或同等学历）平均为$ 200,000/年/模块。这意味着CMCF实际上可以期望NIH资助的研究人员数量增加，他们需要访问CMCF的NIH资助研究。在2006年，我们计划将LSM 410和511M保持使用，以满足增加研究人员的需求。但是，我们只有少数用户愿意使用该工具的有限能力。相反，LSM 510m几乎持续使用。将一名技术人员添加到工作人员中，以便我们现在有两个知识渊博的人在工作日的7:30 am至7:30 pm（或以后在特殊情况下）为调查人员提供帮助。在授予死线和会议之前，通常需要4-5天的等待LSM 510m。因此，必须提高我们的能力。我们对LSM 710的要求的目标是继续提供最先进的设施，并扩大我们核心的能力，以适应越来越多的NIH用户。自2005年以来，CMCF中的共聚焦使用（HRS）几乎翻了一番，并继续增长，这使得购买LSM 710是至关重要的需求。所需的LSM 710具有类星光谱检测，34个光谱检测通道和6个激光线-405、458、488、514、561和633 nm，以及孵化设备，用户将使用户能够对多流载体进行优化的共同局部化，并可以方便地获得有关Protein Protote互动和蛋白质蛋白质的信息。此提案中详细介绍的项目是从11个主要用户和23个未成年人用户中选择的。