ZEISS META 510 CONFOCAL IMAGING SYSTEM: NEURAL DISEASES AND HIV

ZEISS META 510 共焦成像系统：神经疾病和 HIV

• 批准号: 7334970
• 负责人: JUDITH K CHRISTMAN
• 金额: $ 3.03万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334970
• 关键词: AIDS; biochemistry; biomedical facility; confocal scanning microscopy; lasers; medicine; molecular biology; neoplasm /cancer; protein protein interaction; university; veterans

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): The Department of Biochemistry and Molecular Biology is the home of a shared instrument facility for confocal laser scanning microscopy and is currently equipped with Zeiss LSM 410. This instrument, now almost 10 years old, is used extensively by researchers from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases, the UNMC/Eppley Cancer Center and the Veterans' Administration Hospital in Omaha. The facility is located in the Durham Research Center, a new 10-story basic research facility. This Center contains 136 laboratory modules plus one floor dedicated to core facilities including the Confocal Micorscopy Core. Occupancy of lab modules will be limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging ~$200,000/year/module. Our objective is to continue providing state-of-the-art facilities to enhance the quality of research at UNMC and to expand the capacity of our core to accommodate an increasing pool of users. This will be done through the purchase of a fully motorized confocal LSM system with up-to-date analytic software, lasers and accessories that give maximum resolution and separation of fluorescent emissions and detectors that acquire spectral signatures for each pixel. This will allow the user group at UNMC to perform optimized co- localization, and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects listed in this proposal are selected from those of the 12 major users out of a current total of 19. It is anticipated that the user pool will double over the next 3-5 years with the expansion of the research effort on the UNMC campus through new recruitment and from the continued growth of a cadre of occasional users.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。关于艾滋病描述（由申请人提供）：生物化学和分子生物学系是共享仪器设施的共享仪表式激光扫描显微镜的所在地，目前配备了Zeiss LSM 410。由医学院，Eppley癌症和相关疾病研究所，UNMC/Eppley癌症中心和奥马哈退伍军人管理医院的研究人员。该设施位于新的10层基础研究机构达勒姆研究中心。该中心包含136个实验室模块，以及一层专门针对包括共聚焦Micorscopy Core在内的核心设施。实验室模块的占用将仅限于当前和新招募的研究人员，其积极研究补助金（NIH或同等学历）平均为200,000美元/年/模块。我们的目标是继续提供最先进的设施，以提高UNMC的研究质量，并扩大我们核心的能力以适应越来越多的用户。这将通过购买充分电动的共聚焦LSM系统，具有最新的分析软件，激光器和配件，从而可以最大程度地分辨和分离荧光排放和探测器，并为每个像素获取光谱特征。这将使UNMC的用户组能够执行优化的共同定位，并方便地获取有关活细胞中蛋白质 - 蛋白质相互作用和蛋白质运输的信息。此提案中列出的项目是从目前总计19个主要用户中的12个主要用户中选择的。预计随着未来3 - 5年的用户池在UNMC校园中的研究工作扩展，将在未来3 - 5年内翻一番通过新的招聘以及偶尔用户的干部的持续增长。