ZEISS META 510 CONFOCAL IMAGING SYSTEM: PROSTATE, PANCREATIC CANCER, LYMPHOMA

蔡司 META 510 共焦成像系统：前列腺癌、胰腺癌、淋巴瘤

• 批准号: 7334972
• 负责人: JUDITH K CHRISTMAN
• 金额: $ 14.79万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334972
• 关键词: biochemistry; biomedical facility; confocal scanning microscopy; lasers; lymphoma; medicine; molecular biology; neoplasm /cancer; pancreas neoplasms; prostate; protein protein interaction; university; veterans

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Department of Biochemistry and Molecular Biology is the home of a shared instrument facility for confocal laser scanning microscopy and is currently equipped with Zeiss LSM 410. This instrument, now almost 10 years old, is used extensively by researchers from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases, the UNMC/Eppley Cancer Center and the Veterans' Administration Hospital in Omaha. The facility is located in the Durham Research Center, a new 10-story basic research facility. This Center contains 136 laboratory modules plus one floor dedicated to core facilities including the Confocal Micorscopy Core. Occupancy of lab modules will be limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging ~$200,000/year/module. Our objective is to continue providing state-of-the-art facilities to enhance the quality of research at UNMC and to expand the capacity of our core to accommodate an increasing pool of users. This will be done through the purchase of a fully motorized confocal LSM system with up-to-date analytic software, lasers and accessories that give maximum resolution and separation of fluorescent emissions and detectors that acquire spectral signatures for each pixel. This will allow the user group at UNMC to perform optimized co- localization, and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects listed in this proposal are selected from those of the 12 major users out of a current total of 19. It is anticipated that the user pool will double over the next 3-5 years with the expansion of the research effort on the UNMC campus through new recruitment and from the continued growth of a cadre of occasional users.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：生物化学与分子生物学系拥有共焦激光扫描显微镜共享仪器设施，目前配备蔡司 LSM 410。该仪器已有近 10 年历史，被研究人员广泛使用来自医学院、埃普利癌症及相关疾病研究所、UNMC/埃普利癌症中心和奥马哈退伍军人管理局医院。该设施位于达勒姆研究中心，这是一座新的 10 层基础研究设施。该中心包含 136 个实验室模块以及一层专用于核心设施（包括共焦显微镜核心）的楼层。实验室模块的占用仅限于当前和新招募的研究人员，这些研究人员拥有平均每年/模块约 200,000 美元的活跃研究经费（NIH 或同等经费）。我们的目标是继续提供最先进的设施，以提高 UNMC 的研究质量，并扩大我们的核心容量，以适应​​不断增加的用户群体。这将通过购买全电动共焦 LSM 系统来完成，该系统配备最新的分析软件、激光器和配件，可提供最大分辨率和荧光发射分离，以及获取每个像素光谱特征的探测器。这将使 UNMC 的用户组能够执行优化的共定位，并方便地获取有关活细胞中蛋白质-蛋白质相互作用和蛋白质运输的信息。本提案中列出的项目是从目前总共 19 个主要用户中选出的。预计随着 UNMC 校园研究工作的扩大，用户池将在未来 3-5 年内翻一番。通过新的招募和临时用户队伍的持续增长。