MHC Genetic Typing

MHC基因分型

• 批准号: 10217280
• 负责人: CARLOS A SARIOL
• 金额: $ 28.06万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217280
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Address; Alleles; Animal Husbandry; Animals; Arizona; Back; Biochemistry; Biological Models; Biomedical Research; Breeding; Caribbean region; Caring; Cellular Immunity; Collaborations; Communities; DNA laboratory; Databases; Development; Disease; Disease Progression; Ensure; Excision; Foundations; Funding; Generations; Genetic; Genetic Heterogeneity; Genetic Variation; Genome; Goals; HIV; Health; Heterozygote; Human; Immune; Immune response; Inbreeding; Individual; Institution; International; Island; Macaca; Macaca mulatta; Maintenance; Major Histocompatibility Complex; Modeling; Monkeys; Natural Sciences; Physiological; Physiology; Population; Population Genetics; Primates; Production; Proteins; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Roundup; SIV; Safety; Schools; Shapes; Stratification; Testing; Time; United States National Institutes of Health; Universities; Virus; animal colony; base; computerized; design; driving force; genetic analysis; genetic pedigree; germ free condition; human disease; human subject; improved; intergenerational; mathematical sciences; microbicide; nonhuman primate; operation; programs; relational database; research and development; research facility; therapeutic development; virology

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. This specific core is designed to continue our current efforts to maintain a genetically diverse, MHC-defined SPF Indian-origin rhesus macaque colony for use in AIDS-related research. In 2015, the KDL began its collaboration with the current U42 to design and improve the SPF breeding colony to enhance the efficiency of colony maintenance as well as to maximize genetic heterogeneity of the colony animals. Since then we have been constructing multigenerational SPF pedigrees at the CPRC which can be traced back to founders in the Cayo Santiago island population.

项目摘要/摘要： 该应用的主要目的是维持加勒比海的特定病原体（SPF）计划 灵长类动物研究中心（CPRC）通过支持操作，管理，兽医，病毒学 和MHC打字，并通过改进我们的全面遗传管理计划。一个独特的功能 CPRC动物有利于成功建立和可持续的SPF殖民地的动物是这些动物是 自然没有SIV和SRV-D，这是要测试的四种病毒中的两种。然而，出于安全和要求 对于SPF计划，我们继续系统地测试所有四种病毒（B病毒，STLV，SIV和SRV-D）。这 计划提供具有固定的遗传和病毒学背景的无混合的印度王朝恒河猴子。 人口遗传分析表明，随着时间的流逝，SPF菌落在遗传上保持均匀 其非SPF创始人的足够杂合性和最小的分层。遗传 使用此处描述的遗传管理策略将在SPF菌落中保持差异 应用。遗传管理方法将允许富集需求中​​所需的MHC标记 进行生物医学研究，同时保持遗传多样性。该特定核心旨在继续我们的当前 为维持遗传多样的，MHC定义的SPF印度恒河猕猴菌落的努力用于 与艾滋病有关的研究。 2015年，KDL开始与当前的U42合作，以设计和改进 SPF育种菌落以提高菌落维持的效率以及最大化遗传 菌落动物的异质性。从那以后，我们一直在建造多代SPF谱系 可以追溯到圣地亚哥岛人口中的创始人的CPRC。