ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM

加强 CPRC-SPF 恒河猴计划

• 批准号: 8502561
• 负责人: CARLOS A SARIOL
• 金额: $ 236.79万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8502561
• 关键词: Acquired Immunodeficiency Syndrome; Animal Housing; Animals; Area; Biological Assay; Biomedical Research; Birth; Breeding; Caribbean region; Characteristics; Communities; Country; Development; Funding; Genetic; Genetic Heterogeneity; Genetic Markers; Grant; Growth; Housing; Human; Inbreeding; Macaca; Macaca mulatta; Medical; Modeling; Monitor; Monkeys; National Center for Research Resources; Papovaviridae; Polyomavirus; Primates; Provider; Puerto Rico; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Science; Serologic tests; Serological; Simian T-lymphotropic virus 1; Simian virus 40; Specific Pathogen Frees; Structure; Surveys; Techniques; Testing; United States National Institutes of Health; Universities; Virus; cohort; germ free condition; meetings; offspring; programs; vector vaccine; virology; Acquired Immunodeficiency Syndrome; Animal Housing; Animals; Area; Biological Assay; Biomedical Research; Birth; Breeding; Caribbean region; Characteristics; Communities; Country; Development; Funding; Genetic; Genetic Heterogeneity; Genetic Markers; Grant; Growth; Housing; Human; Inbreeding; Macaca; Macaca mulatta; Medical; Modeling; Monitor; Monkeys; National Center for Research Resources; Papovaviridae; Polyomavirus; Primates; Provider; Puerto Rico; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Science; Serologic tests; Serological; Simian T-lymphotropic virus 1; Simian virus 40; Specific Pathogen Frees; Structure; Surveys; Techniques; Testing; United States National Institutes of Health; Universities; Virus; cohort; germ free condition; meetings; offspring; programs; vector vaccine; virology

DESCRIPTION (provided by applicant): The objective of this proposal is to maintain and enhance the specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Caribbean Primate Research Center (CPRC). This program consists of two SPF colonies (one closed and the other open). While the closed colony growths only by births, the open colony increases its size not only by births, but also by incorporating SPF candidates to its cohort. The closed colony (referred as to the U24 SPF colony) will consist of a minimum of 652 animals, and the open colony (referred as to SPF-A) will start with 468 animals. Thus, the founding stock of these genetically-characterized Indian-origin monkey colonies will be donated by CPRC and will consist of approximately 1,120 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques. About 82 (7%) of the U24 SPF animals will be also free of the papovavirus SV40. Thus, this program will consist of two U24 SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these SPF sub colonies will be maintained in well-defined and separated areas, will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. All SPF colonies will be closely monitored by well-established genetic and virology techniques and assays. A panel of highly polymorphic genetic markers will be used to characterize the U24 SPF and SPF-A colonies and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. At the end of this four year grant, the SPF-A colony will be closed. The entire program will produce a total estimated offspring of more than 1,350 SPF rhesus macaques, of which 96 will be also SV40 negative. It is expected that the SPF colonies of this program will make available to PHS-funded grants about 300 animals/year and that it will attain self-sufficiency by the end of this grant. CPRC SPF macaques will help meet the increased research demand that exists for these animals. Our Center will continue to make significant contributions to AIDS and to other research programs by providing high quality, healthy, and genetically-characterized SPF rhesus monkeys to NIH-sponsored research initiatives.

描述（由申请人提供）：该提案的目的是维护和增强加勒比灵长类动物研究中心（CPRC）的特定无病原体（SPF）猕猴供应和育种菌落计划。该程序由两个SPF殖民地组成（一个关闭，另一个开放）。虽然封闭的殖民地仅通过出生而增长，但开放式菌落不仅增加了大小，而且还通过将SPF候选人纳入同伙来增加。封闭的菌落（称为U24 SPF菌落）至少由652只动物组成，开放菌群（称为SPF-A）将以468只动物开始。因此，这些遗传特征的印度猴子菌落将由CPRC捐赠，由大约1,120 SPF（疱疹B，STLV-1，SIV，SIV和SRV无鼠尾草）捐赠。大约82名U24 SPF动物也将不含帕波维瓦病毒SV40。因此，该程序将由两个U24 SPF子菌落组成：一个无SV40-FROE，另一个SV40阳性。来自这些SPF子菌落的动物将保持在明确和分离的区域，将饲养在繁殖玉米中，并将每季度测试上述目标病毒。所有SPF菌落都将受到建立良好的遗传和病毒学技术和测定的密切监测。一组高度多态遗传标记将用于表征U24 SPF和SPF-A菌落并优化育种基团的结构，从而最大程度地提高其遗传异质性的水平。在这四年赠款的结束时，SPF-A殖民地将关闭。整个程序将产生超过1,350个SPF恒河猕猴的总估计后代，其中96个也是SV40负数。预计该计划的SPF殖民地将为phs资助的大约300只动物提供给300只动物，并在这笔赠款结束时获得自给自足。 CPRC SPF猕猴将有助于满足这些动物的研究需求增加。我们的中心将通过为NIH赞助的研究计划提供高质量，健康和遗传特征的SPF恒河猴，继续为艾滋病和其他研究计划做出重大贡献。