MECHANISMS OF HEALTH EFFECTS OF EXERCISE IN CHILDREN

运动对儿童健康影响的机制

• 批准号: 7956531
• 负责人: PIETRO R GALASSETTI
• 金额: $ 0.8万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956531
• 关键词: Asthma; Biological Assay; Cell Line; Child; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Exercise; Fluorescence; Funding; Glucose; Grant; Growth Factor; HL60; Heat-Shock Proteins 70; Heating; Human; Immune; Institution; Insulin; Laboratories; Lipids; Metabolic syndrome; Molecular; Peroxidases; Physical activity; Proteins; Research; Research Personnel; Resources; Source; Stimulus; United States National Institutes of Health; cytokine; neutrophil

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory is studying human neutrophils and the HL60 promyelocytic cell line to examine the molecular immune and growth factor mechanisms through which physical activity alters diabetes, asthma, and metabolic syndrome in children. At the LFD, we will be performing immunofluorescent assays to determine colocalization, quantification, and mobilization of various proteins (e.g. HSP70, myeloperoxidase, LFA-1) after exercise-like stimuli (e.g. heat, pH alteration) under mock-dysmetabolic conditions (e.g. high glucose/insulin/lipids/cytokines).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们的实验室正在研究人类嗜中性粒细胞和HL60临时细胞细胞系，以检查分子免疫和生长因子机制，从而改变儿童的糖尿病，哮喘和代谢综合征。 在LFD上，我们将进行免疫荧光测定，以确定各种蛋白质（例如HSP70，骨髓过氧化物酶，LFA-1）在运动样刺激（例如热量代表代谢条件下（例如，高glucose/Insulsions/Insuls/per））之后的共定位，定量和动员。