GENETIC AND VIRAL STATUS OF THE SOOTY MANGABEY IN THE IVORY COAST TAI FOREST

科特迪瓦泰森林中乌白白眉猴的遗传和病毒状况

• 批准号: 7958163
• 负责人: JAMES GIBSON ELSE
• 金额: $ 5.67万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958163
• 关键词: African; Animals; Behavior; Cercocebus; Cercocebus atys; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Genes; Genetic; Grant; Incidence; Individual; Institution; Ivory Coast; Laboratory Procedures; Maps; Monitor; Monkeys; Movement; Pattern; Population; Population Genetics; Positioning Attribute; Primates; RNA; RNA analysis; Reproduction; Research; Research Personnel; Resources; Sampling; Shipping; Ships; Source; Training; United States National Institutes of Health; Virus; Virus Diseases; Work; animal colony; forest; member; virus genetics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The focus of this collaborative study is the interaction between behavior, genes, and viruses of sooty mangabeys (Cercocebus atys) found in the West African Tai Forest National Park, Ivory Coast. Over 200 animals comprising two separate troops have been habituated and individually identified and are being monitored daily by trained field technicians. Fecal samples are being collected from known individuals, preserved in RNALater, and shipped to Yerkes for RNA and DNA extraction/analysis. Our aims are twofold. First, we hope to collect samples from a sufficient number of animals to determine the genetic relationships of the troop members in order to investigate movement patterns and reproduction strategies. Second, through RNA analysis, we will assess the incidence of natural simian immuno-deficiency virus infections (SIVsm) in wild mangabeys. Since both the SIV-RNA and the monkey-DNA can be extracted from a single fecal sample, we will then be in a position to map the population genetics of the virus onto that of the monkeys to understand how viruses migrate through monkey populations. To date, we have collected and stored 50 sooty mangabey fecal samples from Tai, and are working to refine our laboratory procedures using colony animal samples, while continuing to collect samples from the field.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这项合作研究的重点是在科特迪瓦西非泰森林国家公园发现的乌白眉猴（Cercocebus atys）的行为、基因和病毒之间的相互作用。 超过 200 只动物组成了两个独立的队伍，它们已经习惯并被单独识别，并由训练有素的现场技术人员每天进行监测。粪便样本是从已知个体中收集的，保存在 RNALater 中，并且 运送到 Yerkes 进行 RNA 和 DNA 提取/分析。 我们的目标是双重的。 首先，我们希望从足够数量的动物身上收集样本，以确定部队成员的遗传关系，以便研究运动模式和繁殖策略。 其次，通过 RNA 分析，我们将评估野生白眉猴中天然猿猴免疫缺陷病毒感染 (SIVsm) 的发病率。 由于 SIV-RNA 和猴子 DNA 都可以从单个粪便样本中提取，因此我们将能够将病毒的群体遗传学映射到猴子的群体遗传学上，以了解病毒如何在猴子中迁移 人口。 迄今为止，我们已从 Tai 收集并储存了 50 个乌黑白眉猴粪便样本，并正在努力利用群体动物样本完善我们的实验室程序，同时继续从实地收集样本。