SPF BREEDING COLONIES-SUPPLEMENT

SPF 育种菌落-补充剂

• 批准号: 8357523
• 负责人: JAMES GIBSON ELSE
• 金额: $ 7.43万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357523
• 关键词: Acquired Immunodeficiency Syndrome; Alleles; Animals; Blood specimen; Breeding; Communicable Diseases; DNA; Ensure; Funding; Genetic; Goals; Grant; Haplotypes; Health Priorities; Housing; Macaca mulatta; Microsatellite Repeats; National Center for Research Resources; Population; Primates; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Source; United States National Institutes of Health; cost; design; expectation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is the SPF Breeding Colony Supplement. Animals are made available as subjects to NIH supported investigators for AIDS related research and contribute to national health priorities. The goal of this project is to obtain more complete information regarding the genetic MHC region, which is vital for all SPF rhesus macaques housed at the Yerkes Primate Center. This information will greatly enhance the utility of these animals for all infectious disease studies undertaken at the Center. This includes haplotype analysis of the MHC region and typing of nine specific functional alleles within the region. To date, we have collected blood samples from the animals for analysis and are currently performing all the necessary DNA extractions. PCR conditions for each microsatellite have been optimized and multimarker panels designed. Preliminary analysis of a small subset of the population has been performed to ensure that expected results were obtained. Specific functional allele typing is currently undergoing optimization with the expectation to perform both aspects of this project as soon as DNA extractions are complete.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 这是 SPF 育种菌群补充剂。动物可作为 NIH 支持的研究人员进行艾滋病相关研究的对象，并为国家卫生优先事项做出贡献。该项目的目标是获得有关 MHC 遗传区域的更完整信息，这对于耶基斯灵长类动物中心饲养的所有 SPF 恒河猴至关重要。这些信息将极大地提高这些动物在该中心进行的所有传染病研究中的效用。这包括 MHC 区域的单倍型分析以及该区域内九个特定功能等位基因的分型。迄今为止，我们已采集了动物的血液样本进行分析，目前正在进行所有必要的 DNA 提取。每个微卫星的 PCR 条件均经过优化并设计了多标记组。已经对一小部分人群进行了初步分析，以确保获得预期结果。特定功能等位基因分型目前正在进行优化，期望在 DNA 提取完成后立即执行该项目的两个方面。