The Biophysics Collaborative Access Team Fiber Diffraction Core)

生物物理学协作访问团队光纤衍射核心）

• 批准号: 10032574
• 负责人: THOMAS C IRVING
• 金额: $ 281.99万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10032574
• 关键词: 20 year old; Address; Amyloid; Animal Model; Archives; Area; Biocompatible Materials; Biological; Biophysics; Collaborations; Communities; Complex; Computer software; Connective Tissue; Crystallography; Data; Data Analyses; Diagnostic; Ensure; Equipment; Experimental Designs; Feedback; Fiber; Funding; Hour; Image; Individual; Infrastructure; International; Maintenance; Metadata; Modality; Modeling; Modernization; Molecular Structure; Muscle; Muscle Contraction; Muscle Proteins; Myocardium; Neoplasm Metastasis; Network Infrastructure; Neurodegenerative Disorders; Peer Review; Performance; Procedures; Publications; Quality Control; Recording of previous events; Regulation; Reproducibility; Resource Development; Resources; Roentgen Rays; Sampling; Scanning; Schedule; Seminal; Site; Skeletal Muscle; Skin; Source; Striated Muscles; Structure; Synchrotrons; System; Technology; Time; Tissues; Training; Transgenic Animals; Traumatic Brain Injury; United States National Institutes of Health; Visit; Work; archive data; beamline; brain tissue; computer infrastructure; data acquisition; data analysis pipeline; data archive; data curation; data infrastructure; data integrity; data management; data quality; data reduction; detector; experience; experimental study; fibrous protein; human disease; imaging study; improved; inherited cardiomyopathy; insight; instrument; instrumentation; muscular system; operation; programs; simulation; software systems

The objective of the Fiber Diffraction Technology Operations Core at BioCAT is to provide the biomedical community access to optimized facilities for fiber diffraction of muscle and fibrous protein systems that are unique in the USA and competitive with all other facilities worldwide. The muscle diffraction program at BioCAT is renowned for its history of addressing important questions regarding the biophysics of muscle contraction and its regulation. More recently, emphasis has shifted to more directly biomedically relevant problems with muscle from transgenic animal models for human disease, including inherited cardiomyopathies. BioCAT is also known for the seminal fiber crystallography studies on the structure of collagenous and amyloid systems. In recent years, there has been a shift from studying individual fibrous protein samples towards spatially- resolved scanning diffraction imaging studies that examine the relationships between molecular structure and tissue organization in complex biological tissues such as brain and connective tissue in order to address maladies such as traumatic brain injury, neurodegenerative diseases and cancer metastasis. Our staff are internationally recognized experts in fiber diffraction able to provide expertise in all aspects of a project from experimental design, execution of the experiments, through to data analysis and interpretation. Staff work closely throughout the experiments with first time users and in the context of formal collaborations involving complicated experiments. For routine experiments by experienced users, staff provide onsite training, 24/7 on- call support and are available for help both before and after scheduled experiments. We present plans to adapt the BioCAT beamline 18ID to exploit the outstanding opportunities presented by the APS upgrade (APS-U). This includes plans to upgrade the main area detector to a state-of-the-art EIGER2 XE 9M pixel array detector with necessary upgrades to networking and computational infrastructure. The 20 years old beamline will be upgraded with modern control systems and diagnostics with continuing routine maintenance and contingency plans to ensure reliable operations. An ultra-high flux wide bandpass X-ray monochromator will be installed in order to maintain our competitive edge with other facilities nationally and worldwide to the benefit of all experimental modalities. User experience, quality and integrity of data will be enhanced by continuing improvements to the BioCAT developed experimental control hardware/software system, BioCon. The MuscleX data reduction and analysis package, also developed at BioCAT, for muscle diffraction, fiber crystallography, and scanning diffraction imaging will be enhanced and integrated into data analysis pipelines to provide real time feedback to users during experiments as well as greatly accelerate time to publication. BioCAT will provide training and access to multi-scale simulations for interpreting dynamic muscle diffraction data. Procedures and infrastructure will be developed and deployed for robust data and meta-data curation and archiving.

BioCAT 光纤衍射技术运营核心的目标是提供生物医学 社区可以使用优化的肌肉和纤维蛋白系统纤维衍射设施，这些设施 在美国独一无二，与全球所有其他设施相比具有竞争力。 BioCAT 的肌肉衍射计划 因其解决有关肌肉收缩生物物理学的重要问题的历史而闻名 及其监管。最近，重点已转向与生物医学更直接相关的问题 来自用于人类疾病（包括遗传性心肌病）的转基因动物模型的肌肉。 BioCAT 是 还因对胶原和淀粉样系统结构的精纤维晶体学研究而闻名。 近年来，研究已经从单个纤维蛋白样本转向空间研究。 解析扫描衍射成像研究，检查分子结构和 复杂生物组织（例如大脑和结缔组织）中的组织组织，以解决 脑外伤、神经退行性疾病和癌症转移等疾病。我们的员工是 国际公认的光纤衍射专家能够提供项目各个方面的专业知识 实验设计、实验执行、数据分析和解释。员工工作 在与首次用户的实验中以及在正式合作的背景下密切关注 复杂的实验。对于经验丰富的用户的常规实验，工作人员提供现场培训，24/7 在线 致电支持人员，并在预定实验之前和之后提供帮助。我们提出适应计划 BioCAT 光束线 18ID 可以利用 APS 升级 (APS-U) 带来的绝佳机会。 其中包括计划将主区域探测器升级为最先进的 EIGER2 XE 9M 像素阵列探测器 对网络和计算基础设施进行必要的升级。 20 年历史的光束线将是 通过现代控制系统和诊断进行升级，并进行持续的日常维护和应急处理 计划以确保可靠运行。超高通量宽带通 X 射线单色仪将安装在 为了保持我们与国内外其他机构的竞争优势，造福所有人 实验方式。通过持续不断的努力，用户体验、数据质量和完整性将得到增强 对 BioCAT 开发的实验控制硬件/软件系统 BioCon 的改进。这 MuscleX 数据缩减和分析包，也是在 BioCAT 开发的，用于肌肉衍射、纤维 晶体学和扫描衍射成像将得到增强并集成到数据分析流程中 在实验过程中向用户提供实时反馈，并大大加快发布时间。 BioCAT 将提供培训和多尺度模拟的访问，以解释动态肌肉衍射 数据。将开发和部署程序和基础设施以实现强大的数据和元数据管理 并归档。