The Biophysics Collaborative Access Team

生物物理学协作访问团队

基本信息

批准号：
10561672
负责人：
THOMAS C IRVING
金额：
$ 121.67万
依托单位：
ILLINOIS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10561672
关键词：
Address Advisory Committees Aging Amyloid Automation Biochemical Biocompatible Materials Biological Biology Biophysics Biotechnology Cardiomyopathies Cell physiology Cells Charge Collaborations Communities Complex Computer software Connective Tissue Data Data Analyses Dedications Development Disease model Drug Design Educational workshop Ensure Equilibrium Equipment Evolution Fiber Funding Grant Human Pathology Illinois Image Individual Infrastructure Instruction Laboratories Leadership Ligands Light Maintenance Mission Modification Molecular Molecular Biology Molecular Sieve Chromatography Muscle Myocardium National Center for Research Resources National Institute of General Medical Sciences Neoplasm Metastasis Noise Nucleic Acid Folding Nucleic Acids Pathology Persons Photons Physics Physiological Policies Principal Investigator Productivity Proteins Publications Radiation induced damage Recruitment Activity Research Research Personnel Research Project Grants Research Technics Resources Sampling Scanning Schedule Science Shapes Signal Transduction Site Skeletal Muscle Source Structure Students Synchrotrons System Technology Time Tissue Sample Training Training Activity Traumatic Brain Injury United States National Institutes of Health Update Visit X ray diffraction analysis base beamline biological systems biophysical properties community organizations computerized data processing data acquisition data quality experience experimental study fibrous protein improved inherited cardiomyopathy instrumentation interest macromolecule operation outreach prevent professor programs protein structure repaired structural biology symposium synchrotron radiation tool training opportunity user-friendly web site

项目摘要

The mission of the Biophysics Collaborative Access Team (BioCAT) is to provide user access to a state-of-the- art synchrotron beamline (18ID) for diffraction and scattering of non-crystalline biological materials at the Advanced Photon Source, Argonne National Lab. Continued evolution of the beamline since first light in 1997 has resulted in a unique in the USA fiber diffraction program, and a world-class Bio-SAXS program with unique capabilities both in the equilibrium and time-resolved realms. The use of size exclusion chromatography in line with the SAXS camera has ensured unprecedentedly high data quality even with biochemically challenging samples otherwise thought to be intractable for SAXS. The recent incorporation of MALS and DLS in line with SEC allows additional biophysical characterization that can be invaluable in interpreting SAXS data from difficult systems. Our new coflow sheath flow sample cell has revolutionized SAXS data acquisition by preventing radiation damage, enhancing efficiency accompanied by significant increases in signal to noise. BioCAT maintains specialized apparatuses for combined time-resolved X-ray diffraction and physiological studies of cardiac and skeletal muscle. These studies have extended in scope from basic biophysical problems to address new disease models including cardiomyopathies. A traditional strength is fiber diffraction of general fibrous protein systems that has recently expanded into scanning diffraction imaging of fibrous protein structures in complex tissue samples. A major strength of the resource is the experienced and dedicated scientific staff who assist users seven days a week in data acquisition, processing, analysis and interpretation. BioCAT is directed by Dr. Thomas Irving, who has more than 20 years of experience overseeing synchrotron facilities for non-crystalline diffraction and scattering. Dr. Jesse Hopkins oversaw installation of the new coflow SAXS sample cell, established new user-friendly GUI based data acquisition interfaces, and provide advanced tools to interpret SEC-SAXS data. Dr. Hopkins develops and maintains the BioXTAS RAW SAXS data processing and analysis software package and will extend it to allow increased automation to optimize productivity while minimizing on-site effort for users. Dr. Srinivas Chakravarthy BioCAT’s resident biochemist coordinates the SAXS user program, assists users with conducting experiments on site, and also maintains the biochemical infrastructure. Dr. Weikang Ma is an expert in fiber diffraction of muscle and is responsible for providing comprehensive support to all our fiber diffraction users. All four of these individuals are involved in training and dissemination activities, including scientific publications, presentations at conferences, organization of BioCAT workshops and participating in community organized workshops. BioCAT has an updated website with a comprehensive set of instructions for all aspects of the resource that are sufficient for a user to plan and execute successful experiments at the beamline. Together the SAXS and Fiber Diffraction user programs host 70-90 user groups per year and consistently produce 30-40 publications per year.

生物物理学协作访问团队 (BioCAT) 的使命是为用户提供最先进的访问权限艺术同步加速器光束线 (18ID)，用于非晶体生物材料的衍射和散射先进光子源，阿贡国家实验室自 1997 年首次出现以来光束线不断发展。产生了美国独一无二的光纤衍射方案，以及世界一流的 Bio-SAXS 方案，具有独特的平衡和时间分辨领域的能力在线尺寸排阻色谱的使用。即使在具有生化挑战性的情况下，使用 SAXS 相机也能确保前所未有的高数据质量原本被认为很难用 SAXS 处理的样本最近结合了 MALS 和 DLS，符合 SEC 允许额外的生物物理表征，这对于解释 SAXS 数据非常有价值我们的新型 coflow 鞘流样品池彻底改变了 SAXS 数据采集防止辐射损伤，提高效率，同时显着提高信噪比。 BioCAT 组合维护用于时间分辨 X 射线衍射和生理学的专用设备心脏和骨骼肌的研究扩大了基本生物物理问题的范围。解决包括心肌病在内的新疾病模型的传统优势是一般纤维衍射。纤维蛋白系统最近已扩展到纤维蛋白的扫描衍射成像该资源的主要优势是经验丰富且专注。科学人员每周 7 天协助用户进行数据采集、处理、分析和解释。 BioCAT 由 Thomas Irving 博士领导，他拥有 20 多年监督同步加速器的经验 Jesse Hopkins 博士负责监督新 coflow 的安装。 SAXS样品池，建立了新的用户友好的基于GUI的数据采集界面，并提供先进的 Hopkins 博士开发并维护 BioXTAS RAW SAXS 数据。处理和分析软件包，并将对其进行扩展，以提高自动化程度以进行优化提高生产力，同时最大限度地减少用户的现场工作量。Srinivas Chakravarthy 博士是 BioCAT 的常驻生物化学家。协调SAXS用户程序，协助用户进行现场实验，并维护马伟康博士是肌肉纤维衍射方面的专家，负责为我们所有的光纤衍射用户提供全面的支持所有这四个人都参与其中。培训和传播活动，包括科学出版物、会议演讲、组织 BioCAT 研讨会并参加社区组织的研讨会 BioCAT 有一个更新了网站，提供了一套针对资源各个方面的全面说明，足以满足用户可以将 SAXS 和光纤衍射一起在光束线上规划和执行成功的实验。用户计划每年持续接待 70-90 个用户组，每年出版 30-40 种出版物。