PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

灵长类动物遗传分析和谱系管理

• 批准号: 8357419
• 负责人: JAMES GIBSON ELSE
• 金额: $ 7.43万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357419
• 关键词: Acquired Immunodeficiency Syndrome; Animal Experimentation; Animals; Breeding; Cercocebus; Chromosomes; DNA; Data; Data Set; Databases; Funding; Genetic; Genetic Markers; Genotype; Grant; Macaca; Macaca mulatta; Microsatellite Repeats; National Center for Research Resources; Primates; Principal Investigator; Records; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Source; System; United States National Institutes of Health; Veterinarians; cost; genetic analysis; genetic pedigree; meetings; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Samples for DNA extraction to determine parentage have been collected from over 2000 rhesus macaque, comprising the Yerkes' breeding colony. This includes animals from both the SPF colonies dedicated to AIDS research, and the animals from the NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the authosomal chromosomes. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, to develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variable on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage, and view an animal's record in a single query.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 从2000多个恒河猕猴中收集了用于确定父母的DNA提取的样品，其中包括Yerkes的繁殖菌落。这包括来自专门用于艾滋病研究的SPF菌落和NSPF动物的动物的动物。这些样品中的每一个都针对19种高度多态微卫星的基因分型，分布在整个尸体染色体中。这些数据用于确定我们在现场站维护的所有猕猴和芒果的亲属，谱系和选定的遗传标记。表征特定遗传成分的能力使我们能够更好地满足特定研究者的需求，开发更多样化的研究工作，有选择地培养特定的遗传特征，并进行特定的表型比较。为了管理此数据集，我们正在建立一个全尺度数据库系统，该系统将能够在所有动物上吸收遗传，父母，血统和人口统计学变量。该数据库系统将被整合到我们当前的动物记录系统中，这将使Yerkes的调查人员和兽医能够在单个查询中跟踪，管理和查看动物的记录。