NANOVECTORS

纳米向量

• 批准号: 7721168
• 负责人: MAURO FERRARI
• 金额: $ 1.62万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721168
• 关键词: Architecture; Carbon Nanotubes; Computer Retrieval of Information on Scientific Projects Database; Condition; Contrast Media; Coronary Arteriosclerosis; Detection; Disease; Endothelial Cells; Faculty; Functional disorder; Funding; Grant; Hemorrhage; Image; In Vitro; Institution; Liposomes; Malignant Neoplasms; Molecular; Pathogenesis; Play; Quantum Dots; Research; Research Personnel; Resources; Role; Sampling; Shapes; Silicon; Site; Source; Staging; Students; Surface; System; Therapeutic; Therapeutic Agents; United States National Institutes of Health; Vascular Endothelial Cell; Wound Healing; design; in vivo; mathematical model; mouse model; nanoparticle; nanovector; particle; research study; size; uptake; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Abnormal vasculature architecture and endothelial dysfunction play a fundamental role in the pathogenesis of many diseases including cancer, coronary artery disease, wound healing and hemorrhage. Our strategy to overcome these diseases that are associated with pathological changes in vasculature is a multi-stage nanovector delivery system that comprises of 1st stage porous vector that is loaded with the 2nd stage delivery carriers containing payload. More specifically, the 1st stage vector will selectively target the vascular endothelial cells whose surface has been modified at molecular and cellular level due to the pathological hemodymanic and vasculature maturation, and release the 2nd stage deliver carrier (liposome) at the targeting site, this highly concentrated payload (therapeutic or contrast agent) for the treatment and detection of these disease. In this proposal, we will design and refine the 1st stage nanovector to enhance the ability of targeting to diseased vascular endothelial cells by using mathematical modelling and flow chamber which will be designed and manufactured to mimic each pathological condition. Subsequently, the 2nd stage delivery carrier that contains therapeutic agent will be loaded into 1st stage nanovector, delivered to the pathological vasculature, and released from the 1st stage nanovector to exert their therapeutic action in vitro flow chamber system as well as in vivo mouse model. In the initial experiment, we will examine both large pores(LP) and small pores (SP) samples include 1) silicon particles only, 2) particles loaded with quantum dots, 3) particles loaded with SW carbon nanotubes. One sample (LP) is loaded with both quantum dots and carbon nanotubes. We will image the particle's interaction with and uptake by endothelial cells (HUVEC). The silicon microparticles (LP and SP) are manufactured and characterized by our research faculty, staff and students. Size and shape of these particles are controllable. The 2nd stage nanoparticles (liposomes, quantum dots, carbon nanotubes) are either prepared in our lab, provided by collaborators, or purchased commercially.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 异常的脉管结构和内皮功能障碍在许多疾病的发病机理中起着基本作用，包括癌症，冠状动脉疾病，伤口愈合和出血。 我们克服与脉管系统病理变化相关的这些疾病的策略是一种多阶段的纳米植物输送系统，由第一阶段的多孔向量组成，并带有第二阶段递送载体，其中包含有效载荷。 更具体地说，第一阶段载体将选择性地靶向由于病理血流良好和血管成熟而在分子和细胞水平上经过修饰的血管内皮细胞，并在靶向部位释放第二阶段送货载体（Liposome），这种高度浓缩的有效负荷（治疗或对比剂）（治疗或对比剂）对这些疾病进行了疾病和鉴定。 在此提案中，我们将设计和完善第一阶段的纳米植物，以通过使用数学建模和流动室来增强对患病血管内皮细胞的靶向能力，该模型和流动室将设计和制造以模仿每个病理状况。随后，含有治疗剂的第二阶段输送载体将被加载到第一阶段的纳米植物中，输送到病理脉管系统中，并从第一阶段的纳米窝释放，以在体外流量腔室系统以及体内小鼠模型中发挥其治疗作用。 在最初的实验中，我们将检查大孔（LP）和小孔（SP）样品包括1）仅硅颗粒，2）装有量子点的颗粒，3）装有SW碳纳米管的颗粒。 一个样品（LP）加载了量子点和碳纳米管。我们将图像内皮细胞（HUVEC）与粒子与吸收的相互作用。 硅微粒（LP和SP）的制造和特征是我们的研究教职员工和学生。这些颗粒的大小和形状是可控的。第二阶段的纳米颗粒（脂质体，量子点，碳纳米管）在我们的实验室中由合作者提供，或者在商业上购买。