Small Animal Bone Phenotyping Core

小动物骨表型核心

• 批准号: 7622628
• 负责人: TIMOTHY R NAGY
• 金额: $ 9.91万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7622628
• 关键词: Animal Housing; Animal Model; Animals; Area; Arts; Biology; Bone Diseases; Bone Mineral Contents; Cell Differentiation process; Cellular biology; Complement; Consultations; Core Facility; Data; Development; Development Plans; Diet; Dual-Energy X-Ray Absorptiometry; Education; Ensure; Experimental Designs; Feasibility Studies; Funding Mechanisms; Generations; Goals; Human Resources; Imaging Techniques; In Vitro; Individual; Interdisciplinary Study; Laboratories; Maintenance; Measurement; Measures; Metabolic Bone Diseases; Methods; Mission; Modeling; Operating System; Operative Surgical Procedures; Osteoporosis; Phenotype; Procedures; Publications; Quality Control; Research; Research Personnel; Resources; Rheumatoid Arthritis; Roentgen Rays; Role; Services; Techniques; Technology; Testing; Time; Validation; X-Ray Computed Tomography; bone; bone cell; bone imaging; bone loss; cost; density; gene therapy; in vivo; innovation; instrument; instrumentation; method development; novel; nutrition; quality assurance; ranpirnase; response; validation studies

The ability to rapidly screen animals for bone mineral content (BMC) and density (BMD) is of great importance. Recent advances in instrumentation, including dual-energy X-ray absorptiometry (DXA) and micro-computed tomography (uCT) have made it possible to determine BMC and BMD in vivo in small animal models. The proposed Small Animal Bone Phenotyping Core is a unique facility that has been developed by the Director, Dr. Timothy Nagy, to enable single projects to access state-of-the-art imaging techniques for the determination of BMC and BMD. The Core will provide all resources, including instrumentation and personnel, to carry out its mission. A centralized Core is required due to the cost of the instruments and the expertise required in their operations. The Director and personnel of the Core have demonstrated expertise in the effective use of this technology for the measurement of BMC and BMD in small animals, and are at the forefront of method development and validation of methods in this area. The Core will operate according to a formal quality assurance plan. In addition, the Core will provide extensive education in terms of the appropriate approaches, the limitations of the technology, and the interpretation of data. Extensive consultation on experimental design (especially animal housing and diet) also will be provided to ensure the generation of informative data. Two specific aims are proposed for this interdisciplinary Core: Specific Aim 1: To provided accurate and precise measures of bone mineral content and density in small animal models. This will be accomplished by providing a centralized core facility containing stateof- the-art instrumentation (DXA and uCT) for in vivo and ex vivo bone imaging. Specific Aim 2: Validate new techniques and procedures that will enhance research in bone biology and allow investigators to ask novel questions while at the same time being assured of the resultant data. The Core has been strategically developed in response to investigator demand and has in the past 4 years supported 20 projects instigated by 13 past or current CCBSR investigators, including four Pilot and Feasibility study investigators. It has already stimulated innovative studies in the areas of basic bone cell biology as well as interdisciplinary studies in the area of the development of gene therapy for bone diseases. It is anticipated that its role in the CCBSR will expand considerably over the next 5 years given the enhanced access provided by the P30 funding mechanism, and the increasing use of small animal models by the CCBSR investigators. The Core complements the efforts of the other two CCBSR research Cores and several investigators use all three Cores in their ongoing studies.

快速筛选动物骨矿物质含量 (BMC) 和密度 (BMD) 的能力非常重要 重要性。仪器的最新进展，包括双能 X 射线吸收测定法 (DXA) 和 微计算机断层扫描 (uCT) 使得在小范围内测定体内 BMC 和 BMD 成为可能 动物模型。拟议的小动物骨表型分析核心是一个独特的设施，已被 由主任 Timothy Nagy 博士开发，使单个项目能够获得最先进的成像 测定 BMC 和 BMD 的技术。核心将提供所有资源，包括 仪器和人员，以执行其任务。由于成本的原因，需要一个集中式核心 其操作所需的仪器和专业知识。核心董事及人员 已展现出有效利用该技术测量 BMC 和 BMD 的专业知识 在小动物中，并且处于该领域方法开发和方法验证的前沿。 核心将根据正式的质量保证计划运行。此外，核心还将提供 在适当的方法、技术的局限性和 数据解释。关于实验设计（特别是动物饲养和饮食）的广泛咨询 还将提供以确保生成信息数据。为此提出了两个具体目标 跨学科核心： 具体目标 1：提供准确、精确的骨矿物质含量和密度测量 小动物模型。这将通过提供一个包含 stateof- 的集中式核心设施来实现 用于体内和离体骨成像的最先进仪器（DXA 和 uCT）。 具体目标 2：验证可加强骨骼研究的新技术和程序 生物学，并允许研究人员提出新问题，同时确信 结果数据。 该核心是为了响应研究者的需求而战略性开发的，并且在过去的 4 年里 支持由 13 名过去或现任 CCBSR 调查员发起的 20 个项目，其中包括 4 名试点和 可行性研究调查员。它已经刺激了基础骨细胞领域的创新研究 骨基因治疗开发领域的生物学以及跨学科研究 疾病。预计其在 CCBSR 中的作用将在未来 5 年内大幅扩大 P30 融资机制提供的更多准入，以及小动物的更多使用 CCBSR 研究人员制作的模型。该核心补充了其他两项 CCBSR 研究的成果 核心和几位研究人员在他们正在进行的研究中使用所有三个核心。