Zeiss 510 Multiphoton/single-photon Confocal Microscope

Zeiss 510 多光子/单光子共焦显微镜

• 批准号: 6877387
• 负责人: WARREN R ZIPFEL
• 金额: $ 50万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877387
• 关键词: biological signal transduction; biomedical equipment purchase; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Zeiss 510 multiphoton/single-photon confocal microscope to be housed in the Department of Biomedical Sciences in the Cornell College of Veterinary Medicine. The goal is to provide a modern, user-friendly fluorescence imaging system for use by PHS-funded researchers located in Biomedical Sciences and other departments within the Veterinary College. The microscope will also become part of the newly established Cornell Imaging Core, a campus-wide initiative to provide state-of-art imaging for all biological and biomedical researchers at the Ithaca campus. As part of the core, the instrument will be made available to PHS-funded researchers from other departments on the Cornell campus as scheduling permits. This equipment is required due to an increase in vivo imaging projects at Cornell involving rodent and transgenic mouse models of disease and pathophysiology. In addition, several other PHS-funded projects that require microscopic imaging of cell cultures will also benefit from the multicolor (single photon) confocal imaging capabilities of the Zeiss 510. Researchers from Biomedical Sciences whose projects involve in vivo imaging are currently using the multiphoton imaging systems of Developmental Resource for Biophysical Imaging and Opto-electronics (DRBIO), which is located in the School of Applied and Engineering Physics at Cornell. DRBIO is funded by NIBIB via the P41 mechanism and is a national Resource, and therefore cannot give priority to Cornell researchers over PHS-funded users from other universities. Recently time on DRBIO's three multiphoton microscopes suitable for in vivo imaging projects has become limited due to high demand. For example, DRBIO has collaborators scheduling time as much as three months in advance. In addition, infrastructure demands of live animal imaging, such as adequate ventilation of anesthesia gases and proximity to specialized facilities for animal surgery have become more difficult to meet due to building constraints and new Environmental Health and Safety requirements. Finally, transportation of transgenic animals between DRBIO and the transgenic mouse facility, located a mile away, creates a constant aggravation. Infrastructure and transportation problems will be completely alleviated by placement of a multiphoton imaging system near the transgenic mouse facility in the Veterinary College.

描述（由申请人提供）：该提案要求资金购买蔡司 510 多光子/单光子共焦显微镜，安装在康奈尔兽医学院生物医学科学系。目标是提供一种现代化、用户友好的荧光成像系统，供 PHS 资助的生物医学科学和兽医学院其他部门的研究人员使用。该显微镜还将成为新成立的康奈尔成像核心的一部分，这是一项全校范围内的计划，旨在为伊萨卡校区的所有生物和生物医学研究人员提供最先进的成像。作为核心的一部分，该仪器将在时间安排允许的情况下提供给康奈尔大学其他院系的 PHS 资助的研究人员使用。由于康奈尔大学体内成像项目的增加，涉及啮齿动物和转基因小鼠疾病和病理生理学模型，因此需要该设备。此外，其他几个需要对细胞培养物进行显微成像的 PHS 资助项目也将受益于 Zeiss 510 的多色（单光子）共焦成像功能。涉及体内成像项目的生物医学科学研究人员目前正在使用多光子成像生物物理成像和光电子学开发资源系统（DRBIO），位于康奈尔大学应用​​与工程物理学院。 DRBIO 由 NIBIB 通过 P41 机制资助，是国家资源，因此不能优先考虑康奈尔大学的研究人员而不是其他大学由 PHS 资助的用户。最近，由于需求量很大，DRBIO 的三款适用于体内成像项目的多光子显微镜的时间变得有限。例如，DRBIO 的合作者可以提前三个月安排时间。此外，由于建筑限制和新的环境健康和安全要求，活体动物成像的基础设施要求（例如麻醉气体的充分通风和靠近动物手术专用设施）变得更加难以满足。最后，转基因动物在 DRBIO 和一英里外的转基因小鼠设施之间的运输会造成持续的困扰。通过在兽医学院转基因小鼠设施附近放置多光子成像系统，将彻底缓解基础设施和交通问题。

项目成果

The myosin II coiled-coil domain atomic structure in its native environment.

天然环境中的肌球蛋白 II 卷曲螺旋结构域原子结构。

• 发表时间: 2021-04-06
• 影响因子: 11.1
• 作者: Rahmani, Hamidreza;Ma, Wen;Hu, Zhongjun;Daneshparvar, Nadia;Taylor, Dianne W;McCammon, J Andrew;Irving, Thomas C;Edwards, Robert J;Taylor, Kenneth A
• 通讯作者: Taylor, Kenneth A