Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging Core

适用于康奈尔成像核心的宽波长范围 Zeiss 780 NLO/共焦系统

基本信息

批准号：
8734809
负责人：
WARREN R ZIPFEL
金额：
$ 83.44万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-15 至 2015-07-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This high-end S10 proposal requests funds to purchase a Zeiss LSM780 NLO multiphoton/single-photon confocal microscope coupled to a broad tuning femtosecond laser from Newport/Spectra-Physics (the Insight laser). The instrument will be housed in the Cornell Biotechnology Resource Center's (BRC) Imaging Core facility in the basement of Weill Hall on the Ithaca campus of Cornell University. The goal is to provide a modern, user-friendly combined nonlinear and confocal fluorescence imaging system for use by ten NIH-funded major users and 6 minor users located on the Cornell campus. The instrument will replace an outdated Bio-Rad 1024 multiphoton system which was installed originally in 1996 in the P41 Developmental Resource (DRBIO) in collaboration with Bio-Rad, the initial licensee of multiphoton microscopy. The instrument was later taken over by the Cornell Biotechnology Resource Center (BRC) imaging facility which serves the entire campus. It has been retrofitted numerous times, but still runs under the defunct IBM OS/2 operating system and the Bio-Rad LaserSharp software package rendering it completely un-supportable. Acquisition of the state-of-art system requested will greatly advance the capabilities of the Cornell Imaging facility, and allow us to better serve the needs of our NIH funded researchers. This instrument will not only be replacing an unsupportable 16 year old multiphoton microscope, but will be adding several new previously unavailable features that will be of great benefit to our users. These include two-photon excitation wavelengths up to 1300 nm that will provide for deeper imaging with higher viability, and, for the first time, allow our users to assess families o red dyes and fluorescent proteins that are currently inaccessible with our standard Ti:Sapphire source. The system also has the most up-to-date highest quantum efficiency detectors - both in the confocal head and the external non-descanned detector system. All of the users of this proposed microscope will be imaging live animals, tissues or tissue preparations and all will benefit from the 2 to 3 fold increase in fluorescence detection these newer photodetectors provide. Equally important, the Zeiss system and Zen software provide extremely precise control of the laser excitation as required by our users who have a need for 3D localized photoactivation, photobleaching and targeted photoablation. The requested instrument is specifically tailored to the needs of our NIH funded major and minor users, and will provide for their fluorescence imaging needs well into the future.

描述（由申请人提供）：该高端 S10 提案要求资金购买蔡司 LSM780 NLO 多光子/单光子共焦显微镜，该显微镜与 Newport/Spectra-Physics 的宽调谐飞秒激光器（Insight 激光器）相结合。该仪器将安装在康奈尔大学伊萨卡校区威尔厅地下室的康奈尔生物技术资源中心 (BRC) 成像核心设施中。目标是提供一个现代、用户友好的组合非线性和共焦荧光成像系统，供康奈尔大学校园内 10 个 NIH 资助的主要用户和 6 个次要用户使用。该仪器将取代过时的 Bio-Rad 1024 多光子系统，该系统最初于 1996 年与多光子显微镜的最初授权商 Bio-Rad 合作安装在 P41 开发资源 (DRBIO) 中。该仪器后来被康奈尔生物技术资源中心 (BRC) 成像设施接管，为整个校园提供服务。它已经经过多次改造，但仍然在已失效的 IBM OS/2 操作系统和 Bio-Rad LaserSharp 软件包下运行，使其完全不受支持。购买所需的最先进的系统将极大地提高康奈尔大学成像设施的能力，并使我们能够更好地满足 NIH 资助的研究人员的需求。该仪器不仅将取代无法支持的 16 年历史的多光子显微镜，而且还将添加一些以前不可用的新功能，这将对我们的工作大有裨益。用户。其中包括高达 1300 nm 的双光子激发波长，这将提供具有更高可行性的更深成像，并且首次允许我们的用户评估目前使用我们的标准钛宝石无法访问的红色染料和荧光蛋白家族来源。该系统还拥有最新的最高量子效率探测器 - 无论是在共焦头还是外部非退扫描探测器系统中。该显微镜的所有用户都将对活体动物、组织或组织制剂进行成像，并且所有人都将受益于这些新型光电探测器提供的荧光检测能力增加 2 至 3 倍。同样重要的是，Zeiss 系统和 Zen 软件可以根据需要 3D 局部光激活、光漂白和靶向光消融的用户的要求，提供极其精确的激光激发控制。所需的仪器是专门根据 NIH 资助的主要和次要用户的需求量身定制的，并将满足他们未来的荧光成像需求。

项目成果

期刊论文数量（42）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Growing a glue factory: Open questions in laticifer development.

发展胶水工厂：乳胶管开发中的悬而未决的问题。

DOI：
10.1016/j.pbi.2021.102096
发表时间：
2021-08-27
期刊：
Current opinion in plant biology
影响因子：
9.5
作者：
A. Johnson;G. Moghe;Margaret H. Frank
通讯作者：
Margaret H. Frank

A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.

AP2 复合物磷酸化依赖性失活的结构机制。

DOI：
发表时间：
2019-08-29
期刊：
影响因子：
7.7
作者：
Partlow, Edward A;Baker, Richard W;Beacham, Gwendolyn M;Chappie, Joshua S;Leschziner, Andres E;Hollopeter, Gunther
通讯作者：
Hollopeter, Gunther

Phosphatidylserine Externalization Results from and Causes Neurite Degeneration in Drosophila.

磷脂酰丝氨酸外化是果蝇神经突变性的结果和原因。