Surface Analysis Facility for Biomedical Problems

生物医学问题的表面分析设施

基本信息

批准号：
7274282
负责人：
DAVID G CASTNER
金额：
$ 79.04万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-09-01 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7274282
关键词：
Address Area Arts Atomic Force Microscopy Biocompatible Materials Biological Biological Models Biology Biomedical Research Biosensor Calcified Cell surface Cells Chemicals Chemistry Communities Complex DNA DNA Microarray Chip DNA Microarray format Data Analyses Development Devices Educational workshop Electrons Engineering Facility Construction Funding Category Film Foundations Frequencies Generations Health Human Image Image Analysis Immobilization In Vitro Investigation Link Lipids Mechanics Medical Methods Microbial Biofilms Modeling Molecular Molecular Structure Multivariate Analysis Nature Newsletter Oligonucleotides Paper Pathway interactions Peptides Performance Play Polymers Polyurethanes Postdoctoral Fellow Protein Microchips Proteins Protocols documentation Publications Publishing Qualifying Research Research Personnel Research Project Grants Resolution Role Services Site Societies Spatial Distribution Spectrometry, Mass, Secondary Ion Spectrum Analysis Staging Standards of Weights and Measures Structure Students Sum Surface Surface Properties System Techniques Technology Tissue Engineering Training Universities Visit Washington Work biodegradable polymer biomaterial compatibility biomedical facility cell growth day design implantable device in vivo instrument instrumentation interfacial ion source lectures molecular recognition monolayer nanobiology physical property programs research and development spectroscopic imaging technique development training project ultra-high molecular weight polyethylene

项目摘要

DESCRIPTION: (provided by applicant): NESAC/BIO provides the biomedical research community with state-of-the-art surface analysis expertise, instrumentation, experimental protocols, and data analysis methods to address biological and medical problems involving the surface and interfacial regions. Since the nature of the surface strongly influences the composition and recognizability of the biomolecules and cells that interact with materials, understanding the structure of surfaces and the nature of adsorbed biomolecules are key links in understanding interfacial biology for both in vivo (e.g., implanted biomedical devices) and in vitro (e.g., DNA microarrays) applications. ESCA, static SIMS, NEXAFS, SFG and SPM, provide a powerful set of complementary techniques for addressing the challenges and complexity of the new generation of biomaterials and devices being developed with molecular recognition principles. The specific aims of the Technology Research and Development projects are: (1) Surface Analysis Model Systems and Standards, (2) Chemical State Imaging, and (3) Instrument and Technique Development. In the next five years there will be an increased effort at NESAC/BIO on developing instrumentation and techniques for the surface characterization of biosensors (DNA microarrays, protein chips, etc.). Multivariate analysis methods will play an important role in both spectroscopic and image analysis. Standards used in the development of these techniques will include self-assembled monolayers, peptides, and oligonucleotides. The specific aim of the Collaborative Research projects is to use the surface analysis techniques and methods developed in the TRD projects to address important biomedical research problems. The areas of investigation include biosensors, nanobiology, tissue engineering, self-assembled films, cell growth surfaces with peptides, lipid surfaces, DNA arrays, and biomaterials with specific recognition sites. NESAC/BIO service activities will focus on the development and optimization of biomedical devices. Dissemination and Training projects will also be an important part of the NESAC/BIO program.

描述：（由申请人提供）：NESAC/BIO为生物医学研究界提供了最先进的表面分析专业知识，仪器，实验方案以及数据分析方法，以解决涉及地表和界面区域的生物学和医学问题。由于表面的性质强烈影响与材料相互作用的生物分子和细胞的组成和可识别性，因此了解表面的结构以及吸附的生物分子的性质是理解体内生物学界面生物学的关键联系。 ESCA，静态模拟人生，Nexafs，SFG和SPM提供了一套有力的补充技术，以解决具有分子识别原理开发的新一代生物材料和设备的挑战和复杂性。技术研发项目的具体目的是：（1）表面分析模型系统和标准，（2）化学状态成像以及（3）仪器和技术开发。在接下来的五年中，NESAC/BIO在开发生物传感器（DNA微阵列，蛋白质芯片等）表面表征的仪器和技术方面将增加努力。多变量分析方法将在光谱和图像分析中起重要作用。这些技术开发中使用的标准将包括自组装单层，肽和寡核苷酸。协作研究项目的具体目的是使用TRD项目中开发的表面分析技术和方法来解决重要的生物医学研究问题。研究领域包括生物传感器，纳米生物学，组织工程，自组装膜，具有肽的细胞生长表面，脂质表面，DNA阵列和具有特定识别位点的生物材料。 NESAC/BIO服务活动将集中于生物医学设备的开发和优化。传播和培训项目也将是NESAC/BIO计划的重要组成部分。