Integrated Proteom Works System

综合蛋白质工程系统

• 批准号: 6877216
• 负责人: Bryan R. G. Williams
• 金额: $ 22.37万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-16 至 2006-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877216
• 关键词: biomedical equipment purchase; biotechnology; computer program /software; gel electrophoresis; high throughput technology; mass spectrometry; matrix assisted laser desorption ionization; protein quantitation /detection; proteomics; robotics

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a semi-robotic Proteome Works system. This integrated instruments and software package will greatly enhance the Laboratory of Mass Spectrometry with the Lerner Research Institute of the Cleveland Clinic Foundation (CCF) capabilities. The requested integrated protein spot cutter, protein proteolytic digestion/processing/MALDI-spotting station and software will be used to facilitate and advance research programs at institutions within the area (CCF and Case Western Reserve University). Major Users all have numerous NIH-funded research grants, which have component projects that can best be conducted using 2-D gel analysis followed by mass spectrometry. All the Major User's projects require substantial numbers of sample preparations and/or 2-D gel analysis and comparison. The Major Users projects are broad and include: 1) identification of differentially expressed proteins in normal and diseased tissue; 2) quantification of structurally defined components in complex biological complexes; 3) characterization of covalent protein modifications; and 4) investigations of cellular differentiation markers and altered gene expression. The proteome Works system provides the versatility, resolution and capabilities to meet these diverse demands. In addition, the requested instrument system will be used to meet the needs of the occasional user. This service will be conducted within our existing Molecular Biotechnology Core, which provides support to investigators by performing protein sequencing, peptide synthesis, and related services. Due to the human genome project, the Mass Spectrometry Core has experienced greatly increased usage. With the integrated Proteome Works system, the mass Spectrometry Core can process more samples and increase its machine-time efficiency. Importantly, the Proteome Works system will allow users to digitally capture 2-D gel images, compare results of multiple experiments and integrate in gene expression information and relate it directly back to protein identities and expression levels. In fact, the inability to do this has significantly hindered the pace of progress in biomedical research. Acquisition of the Proteome Works system will allow full utilization of the unique capabilities and usefulness of the Mass Spectrometry Core, gene expression laboratories and Cores here at CCF and CWRU. CCF has committed substantial resources to the development of the CCF Laboratory of Mass Spectrometry, including personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term. In sum, acquisition of the requested integrated Proteome Works system will have a significant impact on the progress and future development of biomedical research programs at multiple institutions in the region including CCF and CWRU.

描述（由申请人提供）：本提案的目的是寻求资金购买半机器人 Proteome Works 系统。这种集成仪器和软件包将大大增强克利夫兰诊所基金会（CCF）勒纳研究所的质谱实验室能力。所需的集成蛋白点切割器、蛋白水解消化/处理/MALDI 点样站和软件将用于促进和推进该地区机构（CCF 和凯斯西储大学）的研究项目。主要用户都拥有大量 NIH 资助的研究经费，其中的组成项目最好使用二维凝胶分析和质谱法进行。所有主要用户的项目都需要大量的样品制备和/或二维凝胶分析和比较。主要用户项目范围广泛，包括：1）鉴定正常和患病组织中差异表达的蛋白质； 2) 复杂生物复合物中结构确定的组分的定量； 3) 共价蛋白修饰的表征； 4) 细胞分化标记和基因表达改变的研究。 proteome Works 系统提供了多功能性、分辨率和功能来满足这些不同的需求。此外，所要求的仪器系统将用于满足偶尔用户的需求。这项服务将在我们现有的分子生物技术核心内进行，该核心通过执行蛋白质测序、肽合成和相关服务为研究人员提供支持。由于人类基因组计划，质谱核心的使用量大大增加。借助集成的 Proteome Works 系统，质谱核心可以处理更多样品并提高其机器时间效率。重要的是，Proteome Works 系统将允许用户以数字方式捕获二维凝胶图像，比较多个实验的结果，整合基因表达信息，并将其直接与蛋白质特性和表达水平联系起来。事实上，无法做到这一点已经严重阻碍了生物医学研究的进展步伐。收购 Proteome Works 系统将允许充分利用 CCF 和 CWRU 的质谱核心、基因表达实验室和核心的独特功能和实用性。 CCF 投入了大量资源来发展 CCF 质谱实验室，包括监督其运营的人员，以及确保其长期运营和维护的财政计划。总之，获得所需的集成 Proteome Works 系统将对包括 CCF 和 CWRU 在内的该地区多个机构的生物医学研究项目的进展和未来发展产生重大影响。