Raman Probe for Bronchial Premalignant Lesions

用于支气管癌前病变的拉曼探头

• 批准号: 7053922
• 负责人: STEPHEN FREDERICK FULGHUM
• 金额: $ 9.97万
• 依托单位: NEWTON LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-06 至 2006-09-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7053922
• 关键词: Raman spectrometry; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; bronchoscopy; bronchus neoplasm; clinical biomedical equipment; clinical research; computer assisted diagnosis; computer program /software; fiber optics; fluorescence; human tissue; lung neoplasms; miniature biomedical equipment; neoplasm /cancer diagnosis; preneoplastic state; respiratory imaging /visualization

DESCRIPTION (provided by applicant): The long term objective is a practical clinical Raman instrument with a fiberoptic probe, for use with a bronchoscope, for characterizing bronchial tissue with respect to precancerous conditions. The system will be especially effective with a new generation of bronchoscopes that have their screening efficacy enhanced by use of auto-fluorescence or other spectroscopic imaging methods. Since lung cancer is the second most common cancer in humans, is the most common cause of cancer deaths in the world, and has a very low (14%) five year survival rate, an instrument that can be easily utilized with normal bronchoscopy procedures will add clinical medical value by enabling real time examination of suspicious sights, with enhanced sensitivity relative to white light bronchoscopy alone. This will enable greater yield of "positives" from those sites that are chosen for biopsy. The Specific Aims for Phase I are: (1) The acquisition of Raman spectra at an excitation wavelength of 830 nm from in-vitro samples of normal, dysplastic and cancerous lung tissue, using free-space propagating beams. (2) The production and testing of a small-diameter, filtered, fiberoptic Raman probe, suitable for insertion through the biopsy channel of a bronchoscope. (3) The development of real-time data analysis algorithms which can indicate the state of the observed tissue on the basis of characteristic signatures described in the literature. Basic Raman data will be obtained on in vitro bronchial tissue to assist probe design and algorithm development. Probes will be designed, fabricated and used with a Raman prototype instrument on in vitro bronchial tissue samples to compare with modeling calculations and demonstrate feasibility of a real-time in vivo instrument for use in conjunction with a bronchoscope in clinical examinations.

描述（由申请人提供）：长期目标是一种实用的临床拉曼仪器，带有光纤探头，与支气管镜一起使用，用于表征支气管组织的癌前状况。该系统对于新一代支气管镜尤其有效，通过使用自发荧光或其他光谱成像方法增强了其筛查功效。 由于肺癌是人类第二常见的癌症，是世界上最常见的癌症死亡原因，并且五年生存率非常低 (14%)，因此可以通过正常支气管镜检查程序轻松使用的仪器将通过实时检查可疑景象来增加临床医学价值，并且相对于单独的白光支气管镜检查具有更高的灵敏度。这将使选择进行活检的部位获得更多的“阳性”结果。 第一阶段的具体目标是： (1) 使用自由空间传播光束，从正常、发育不良和癌性肺组织的体外样本中采集激发波长为 830 nm 的拉曼光谱。 (2) 适合插入支气管镜活检通道的小直径、过滤式光纤拉曼探头的生产和测试。 (3)实时数据分析算法的开发，该算法可以根据文献中描述的特征签名来指示所观察组织的状态。将获得体外支气管组织的基本拉曼数据，以协助探针设计和算法开发。探针将被设计、制造并与拉曼原型仪器一起用于体外支气管组织样本，以与建模计算进行比较，并证明实时体内仪器与支气管镜在临床检查中结合使用的可行性。