Spectroscopic Diagnostic for Barrett's Dysplasia

巴雷特发育不良的光谱诊断

• 批准号: 6666929
• 负责人: STEPHEN FREDERICK FULGHUM
• 金额: $ 35.06万
• 依托单位: NEWTON LABORATORIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-04 至 2005-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6666929
• 关键词: Barretts esophagus; biomedical equipment development; cell nucleus; clinical research; computer program /software; computer system design /evaluation; diagnosis design /evaluation; endoscopy; fiber optics; gastrointestinal disorder diagnosis; gastrointestinal imaging /visualization; histopathology; human subject; method development; phantom model; spectrometry

DESCRIPTION (provided by applicant): Barrett's esophagus (BE) is an acquired condition of the lower esophagus in which the normal squamous tissue is replaced by a metaplastic columnar mucosa. BE itself is not malignant, but it is the primary risk factor for esophageal adenocarcinoma, and a significant number of patients with BE will have adenocarcinoma at the time of first endoscopy. Currently, examination for dysplasia requires numerous random biopsy samples. This work will complete the development and clinically test an optical instrument for detecting and grading Barrett's dysplasia. Light scattering spectroscopy (LSS) has already been shown to differentiate between normal and diseased tissue conditions in BE patients, but the analysis is complex so the data is analyzed after the procedure is over. The instrument developed by this work will be based on white light LSS, but it will use a special probe design and data reduction algorithm, developed by NLI, enabling real-time results. The probe design makes use of the properties of light scattering by cell nuclei and other tissue components to enable the separation and subtraction of interfering effects, and the characterization of the nuclei, based on using spectral, angular, and polarization characteristics of the scattering processes. The tissue characterization follows from the instrument's quantitative determination of the nuclear size and number density for the surface mucosal cells - two properties commonly used by pathologists. Specifically, NLI will (i) produce the probe, (ii) calculate the data base for the real-time algorithm, (iii) produce the instrument with light source, spectrometer, and embedded computer (suitable for interface with a standard clinical video endoscope), (iv) verify the instrument operation in the laboratory using standard scattering materials, (v) verify the background subtraction properties by using normal and diseased resected colon tissue, and (vi) conduct clinical studies on patients with Barrett's Esophagus.

描述（由申请人提供）：巴雷特食管（BE）是下食管的后天性疾病，其中正常鳞状组织被化生柱状粘膜取代。 BE本身并不恶性，但它是食管腺癌的首要危险因素，相当一部分BE患者在第一次内镜检查时就会发现腺癌。目前，检查异型增生需要大量随机活检样本。这项工作将完成用于检测和分级巴雷特发育不良的光学仪器的开发和临床测试。光散射光谱 (LSS) 已被证明可以区分 BE 患者的正常组织状况和患病组织状况，但分析很复杂，因此数据在手术结束后进行分析。这项工作开发的仪器将基于白光LSS，但它将使用由NLI开发的特殊探头设计和数据缩减算法，从而实现实时结果。探针设计利用细胞核和其他组织成分的光散射特性，能够分离和减去干扰效应，并基于散射过程的光谱、角度和偏振特性来表征细胞核。组织表征源自仪器对表面粘膜细胞的核大小和数量密度的定量测定——这是病理学家常用的两个属性。具体来说，NLI 将 (i) 生产探头，(ii) 计算实时算法的数据库，(iii) 生产带有光源、光谱仪和嵌入式计算机的仪器（适合与标准临床视频内窥镜接口） ），（iv）使用标准散射材料在实验室中验证仪器操作，（v）使用正常和患病的切除结肠组织验证背景扣除特性，以及（vi）对巴雷特食管患者进行临床研究。