MODELING WOUND HEALING USING A NOVEL SKIN-EQUIVALENT (RAFT) TISSUE CONSTRUCT

使用新型皮肤等效 (RAFT) 组织结构模拟伤口愈合

• 批准号: 7365636
• 负责人: TATIANA B KRASIEVA
• 金额: $ 2.65万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7365636
• 关键词: MODELING; WOUND; HEALING; USING; NOVEL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Wound healing is the process by which the body repairs damaged tissue. Through the work of specialized cells called fibroblasts, connective tissue is regenerated at the site of injury, as fibroblasts produce collagen, fibronectin, elastin, and peptidoglycan to stabilize and heal the wound. Recent studies have shown that in vitro skin-equivalent, collagen-based tissue constructs called RAFTs can be used to model damaged tissue. We developed a model raft culture model based on a collagen/fibronectin gel mixture. Specifically, we constructed a normal collagen-based raft with a circular fibronectin ?plug? in the center to simulate the introduction of a wound into the collagen gel. Fibronectin, unlike collagen, does not generate second-harmonic generation (SHG) signals under multiphoton microscopy (MPM). Thus, the newly synthesized collagen in the fibronectin gel region can be distinguished from the old collagen already present in the outer regions of the construct. This model facilitates the analysis of the overall collagen production and 3-D structure.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。伤口愈合是身体修复组织受损组织的过程。通过称为成纤维细胞的专门细胞的工作，在损伤部位再生结缔组织，因为成纤维细胞产生胶原蛋白，纤连蛋白，弹性蛋白和肽聚糖，以稳定并治愈伤口。最近的研究表明，称为筏子的体外皮肤等效，基于胶原蛋白的组织结构可用于模拟受损的组织。 我们开发了一种基于胶原蛋白/纤连蛋白凝胶混合物的模型筏培养模型。具体来说，我们用圆形纤连蛋白插头构建了一个普通的基于胶原蛋白的筏？在中心，以模拟将伤口引入胶原蛋白凝胶中。与胶原蛋白不同，纤连蛋白不会在多光子显微镜（MPM）下产生第二谐的产生（SHG）信号。因此，可以将纤连蛋白凝胶区域中新合成的胶原蛋白与已经存在于构建体外区域中的旧胶原蛋白区分开。该模型促进了整体胶原蛋白产生和3-D结构的分析。