Single neuronal recordings using movable mircrophobes

使用可移动微镜进行单个神经元记录

基本信息

批准号：
7363641
负责人：
JITENDRAN MUTHUSWAMY
金额：
$ 25.73万
依托单位：
ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7363641
关键词：
Action Potentials Acute Aging Amplifiers Animals Applications Grants Auditory Perception Auditory Physiology Brain Cell Nucleus Chronic Collaborations Condition Decompression Sickness Depth Development Devices Electronics Encapsulated Ensure Evaluation Event Exercise Failure Funding Future Gliosis Goals Grant Head Implant Individual Inflammation Injury Laboratories Lead Length Measures Mechanics Memory Memory impairment Methods Microelectrodes Microfabrication Monitor Movement Necrosis Neurons Numbers Outcome Penetration Pliability Population Positioning Attribute Process Property Prosthesis Protocols documentation Rattus Recovery Research Rodent Signal Transduction Site Staging Stress Stroke Structure Techniques Technology Testing Tissues Translations United States National Institutes of Health Validation awake brain tissue conditioning density design electric impedance electrical potential extracellular implantation improved in vivo memory retrieval models and simulation neurophysiology new technology novel parylene research study response silicon nitride somatosensory transmission process

项目摘要

DESCRIPTION (provided by applicant): Neuroscientists have long recognized the significance of using microelectrode arrays for recording extracellular potentials from populations of neurons, for which a number of such devices have been developed so far. However, current implantable microelectrode technologies to monitor single neuronal function in-vivo often fail in chronic situations, likely due to mechanical drift in positioning mechanisms, micromotion of brain tissue and gliosis around the implant site. The overall goal of the proposal is to develop a reliable technology for recording electrical potentials from single neurons in chronic experiments. We propose to develop a novel microfabricated thermal microactuator and associated microelectrode technology in collaboration with Sandia National Laboratories to enable repositioning of microelectrodes after implantation. The flexibility to reposition the microelectrodes after implantation (in the event of a failure or otherwise) using microactuators will potentially increase the reliability and consistency of single-neuronal recordings in-vivo in chronic experiments with awake and behaving animals. The key goals for developing this technology in this proposal are (a) to enable high quality, reliable single- unit electrical recordings from ensembles of neurons even in deep structures of the brain in awake, behaving rodents (b) to enable reliable positioning and repositioning of microelectrodes in both acute and long-term experiments and (c) assess the effect of microelectrode movement on the surrounding brain tissue. We will use a combination of modeling and simulation, novel microfabrication and packaging techniques, bench-top testing and in-vivo testing approaches for design, characterization and validation. Besides leading to novel discoveries in our own research into neuronal mechanisms of stroke injury and recovery, this new technology will immediately impact several NIH funded grants of our collaborators. The microactuated microelectrode will be tested in a scenario that demands accurate long-term recording from neurons in deep nuclei of the brain. Independent evaluation and dissemination will be ensured with the help of collaborators doing in vivo experiments for understanding the mechanisms of memory retrieval and consolidation and memory deficits in aging, auditory physiology, cortical prostheses etc.

描述（由申请人提供）：神经科学家长期以来已经认识到使用微电极阵列记录神经元种群的细胞外电位的重要性，到目前为止，神经元种群已经开发了许多此类设备。然而，当前可植入的微电极技术监测体内单个神经元功能通常在慢性情况下失败，这可能是由于定位机制的机械漂移，脑组织的微动和植入物周围的胶状病。该提案的总体目标是开发可靠的技术，用于记录慢性实验中单个神经元的电势。我们建议与桑迪亚国家实验室合作开发一种新型的微生物热微型驱动器和相关的微电极技术，以使植入后重新定位微电极。在植入后使用显微厌恶剂的灵活性（如果发生故障或其他），则在慢性实验中，使用微型抗体剂可能会提高单神经元记录的可靠性和一致性。在该建议中开发该技术的主要目标是（a），即使在清醒的大脑深层结构中，从神经元的集合中实现高质量，可靠的单位电气记录，表现啮齿动物（b）以启用急性和长期实验和长期实验和（c）评估Microelectrode的效果的可靠定位和重新定位和重新定位。我们将结合建模和仿真，新颖的微加工和包装技术，台面测试以及维沃测试方法的设计，表征和验证。除了在我们对中风损伤和恢复神经元机制的研究中引起新发现的发现外，这项新技术还将立即影响我们合作者的几项NIH资助的赠款。在需要从大脑深核中进行准确的长期记录的情况下，将测试微型微电极。将在进行体内实验的协作者的帮助下，确保独立评估和传播，以了解记忆检索和巩固的机制，以及衰老，听觉生理学，皮质假体等的记忆缺陷以及记忆缺陷。