Olympus DSU Confocal System

奥林巴斯 DSU 共焦系统

• 批准号: 7044534
• 负责人: Lynn COOLEY
• 金额: $ 13.25万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-15 至 2007-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7044534
• 关键词: Caenorhabditis elegans; Drosophilidae; antibody; cell migration; charge coupled device camera; confocal scanning microscopy; fasting; fluorescence; gene mutation; genetics; housing; kidney; lasers; ligands; light intensity; lighting; mass information media; microscopy; molecular /cellular imaging; mutant; phosphatidylinositols; proteins; receptor; tissue /cell culture; tissues; zebrafish

DESCRIPTION (provided by applicant): The ability to obtain high-resolution, live-cell images and movies is essential for understanding biological processes and how they are perturbed by disease or mutation. This proposal is for the purchase of an Olympus Disc Scan Unit (DSU) confocal microscope system. Unlike laser-scanning confocal microscopy, the Olympus DSU system uses a white light source, thus dramatically reducing the initial cost and maintenance of the system. To compensate for the lower light intensity available from white-light sources, the Olympus DSU system uses a highly sensitive cooled charged-couple device (CCD) camera to capture fluorescence signals. Out-of-focus light is eliminated by the spinning disc with precisely defined slits that create virtual pin-hole illumination. Fast image acquistion (up to 15 frames/sec) and lowered phototoxicity from spinning disc illumination combine to allow high-quality imaging of both fixed and live cells. The Olympus DSU system will be housed in the Department of Genetics and used by investigators for a variety of projects funded by NIH R01 grants. These include imaging of hundreds of GFP-fusion proteins in Drosophila tissues (Cooley), antibody localization in mouse and C. elegans germline cells (Reinke, Sweasy), cell migration in C. elegans (Stern), localization of mutant forms of PDGF receptor in cells treated with ligands (DiMaio), phosphoinositide signaling in cultured cells (H. Sun), and live imaging of developing zebrafish kidneys (Z. Sun). The Olympus DSU system will replace two out-dated, non-motorized fluorescence microscopes that can no longer produce data essential for these projects. It will also provide a much-needed alternative to expensive laser-scanning confocal imaging, especially in cases where the intensity of fluorescence signals is not limiting. Furthermore, the system will allow ready access to time-lapse microscopy, again with a reduced cost compared to laser-based systems.

描述（由申请人提供）：获得高分辨率活细胞图像和电影的能力对于理解生物过程以及它们如何受到疾病或突变的干扰至关重要。该提案旨在购买奥林巴斯圆盘扫描装置 (DSU) 共焦显微镜系统。与激光扫描共焦显微镜不同，奥林巴斯 DSU 系统使用白光源，从而大大降低了系统的初始成本和维护成本。为了补偿白光源的较低光强度，奥林巴斯 DSU 系统使用高灵敏度冷却电荷耦合器件 (CCD) 相机来捕获荧光信号。旋转圆盘具有精确定义的狭缝，可产生虚拟针孔照明，从而消除离焦光。快速图像采集（高达 15 帧/秒）和旋转圆盘照明降低的光毒性相结合，可以对固定细胞和活细胞进行高质量成像。奥林巴斯 DSU 系统将安置在遗传学系，供研究人员用于 NIH R01 拨款资助的各种项目。其中包括果蝇组织中数百个 GFP 融合蛋白的成像 (Cooley)、小鼠和线虫种系细胞中的抗体定位 (Reinke、Sweasy)、线虫中的细胞迁移 (Stern)、PDGF 受体突变形式的定位用配体处理的细胞 (DiMaio)、培养细胞中的磷酸肌醇信号传导 (H. Sun) 以及发育中的斑马鱼肾脏的实时成像 (Z. Sun)。奥林巴斯 DSU 系统将取代两台过时的非机动荧光显微镜，它们不再能生成这些项目所需的数据。它还将为昂贵的激光扫描共焦成像提供急需的替代方案，特别是在荧光信号强度不受限制的情况下。此外，该系统将允许随时使用延时显微镜，与基于激光的系统相比，成本也更低。