OLYMPUS DSU CONFOCAL SYSTEM: ZEBRAFISH:POLYCYSTIC KIDNEY DISEASE

奥林巴斯 DSU 共焦系统：斑马鱼：多囊肾病

• 批准号: 7335305
• 负责人: Lynn COOLEY
• 金额: $ 1.33万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-15 至 2007-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335305
• 关键词: Caenorhabditis elegans; Drosophilidae; antibody; cell migration; charge coupled device camera; confocal scanning microscopy; fasting; fluorescence; gene mutation; genetics; housing; kidney; lasers; ligands; light intensity; lighting; mass information media; microscopy; molecular /cellular imaging; mutant; phosphatidylinositols; polycystic kidney; proteins; receptor; tissue /cell culture; tissues; zebrafish; Caenorhabditis elegans; Drosophilidae; antibody; cell migration; charge coupled device camera; confocal scanning microscopy; fasting; fluorescence; gene mutation; genetics; housing; kidney; lasers; ligands; light intensity; lighting; mass information media; microscopy; molecular /cellular imaging; mutant; phosphatidylinositols; polycystic kidney; proteins; receptor; tissue /cell culture; tissues; zebrafish

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The ability to obtain high-resolution, live-cell images and movies is essential for understanding biological processes and how they are perturbed by disease or mutation. This proposal is for the purchase of an Olympus Disc Scan Unit (DSU) confocal microscope system. Unlike laser-scanning confocal microscopy, the Olympus DSU system uses a white light source, thus dramatically reducing the initial cost and maintenance of the system. To compensate for the lower light intensity available from white-light sources, the Olympus DSU system uses a highly sensitive cooled charged-couple device (CCD) camera to capture fluorescence signals. Out-of-focus light is eliminated by the spinning disc with precisely defined slits that create virtual pin-hole illumination. Fast image acquistion (up to 15 frames/sec) and lowered phototoxicity from spinning disc illumination combine to allow high-quality imaging of both fixed and live cells. The Olympus DSU system will be housed in the Department of Genetics and used by investigators for a variety of projects funded by NIH R01 grants. These include imaging of hundreds of GFP-fusion proteins in Drosophila tissues (Cooley), antibody localization in mouse and C. elegans germline cells (Reinke, Sweasy), cell migration in C. elegans (Stern), localization of mutant forms of PDGF receptor in cells treated with ligands (DiMaio), phosphoinositide signaling in cultured cells (H. Sun), and live imaging of developing zebrafish kidneys (Z. Sun). The Olympus DSU system will replace two out-dated, non-motorized fluorescence microscopes that can no longer produce data essential for these projects. It will also provide a much-needed alternative to expensive laser-scanning confocal imaging, especially in cases where the intensity of fluorescence signals is not limiting. Furthermore, the system will allow ready access to time-lapse microscopy, again with a reduced cost compared to laser-based systems.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：获得高分辨率，活细胞图像和电影的能力对于理解生物学过程以及它们如何受到疾病或突变的扰动至关重要。该建议是用于购买奥林巴斯盘扫描单元（DSU）共聚焦显微镜系统。与激光扫描共聚焦显微镜不同，Olympus DSU系统使用白光来源，从而大大降低了系统的初始成本和维护。为了补偿白色光源可用的较低的光强度，Olympus DSU系统使用了高度敏感的冷却带电组设备（CCD）摄像头来捕获荧光信号。旋转盘以精确定义的缝隙来消除了焦点光，从而产生虚拟针孔照明。快速图像获取（最多15帧/秒），并从旋转盘照明中降低了光毒性，从而可以允许对固定和活细胞进行高质量的成像。 Olympus DSU系统将安置在遗传学系，并被调查人员用于由NIH R01赠款资助的各种项目。 These include imaging of hundreds of GFP-fusion proteins in Drosophila tissues (Cooley), antibody localization in mouse and C. elegans germline cells (Reinke, Sweasy), cell migration in C. elegans (Stern), localization of mutant forms of PDGF receptor in cells treated with ligands (DiMaio), phosphoinositide signaling in cultured cells (H. Sun), and live imaging of developing斑马鱼肾脏（Z. Sun）。 Olympus DSU系统将替换两个较早的非运动荧光显微镜，这些显微镜无法再产生对这些项目必不可少的数据。它还将为昂贵的激光扫描共聚焦成像提供急需的替代方案，尤其是在荧光信号强度不受限制的情况下。此外，与基于激光的系统相比，该系统将允许准备好延时显微镜，成本降低。