MOLECULAR BIOLOGY CORE

分子生物学核心

• 批准号: 7002129
• 负责人: JUANITA L. MERCHANT
• 金额: $ 15.71万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7002129
• 关键词: biomedical facility; gastrointestinal system; laboratory mouse; laboratory rat; microarray technology; model design /development; molecular biology; science education; transfection /expression vector

The purpose of the Molecular Biology core is to facilitate the application of the tools of molecular genetics to the study of gastrointestinal peptides and their physiological functions. In all aspects, the Molecular Biology Core functions to provide essential services and serves as an educational resource to all Center Investigators. Although basic molecular techniques are widely available to Center investigators through commercial sources, there remain specific services that are best provided by pooled resources and by highly trained personnel such as those that can be provided by a Center. During the prior funding period, three Core programs were provided: the Transgenic Animal Program, the Viral Vector Program and the Gene Expression Program. The Molecular Biology Core has added a Microarray Gene Chip Program and has essentially updated the Gene Expression Program to provide education and training in those techniques that can be performed in the investigators own laboratory after receiving some theoretical and practical training. Since investigator needs have likely moved beyond basic gene expression approaches to involve such techniques as chromatin structure and analysis, this primarily education program has been renamed the Molecular Techniques Training Program. Investigators needing assistance with basic molecular techniques, e.g., quantitative RT-PCR and more sophisticated techniques, e.g., chromatin immunoprecipitation assays (ChIP), can receive training from the Core Director (Dr. Merchant) and her associate (Dr. Bai).

分子生物学核心的目的是促进分子遗传学工具的应用 胃肠肽及其生理功能的研究。从各个方面来说，分子 生物学核心的职能是提供基本服务并作为所有中心的教育资源 调查人员。尽管中心研究人员可以通过以下方式广泛使用基本分子技术： 尽管有商业来源，但仍然存在最好由集中资源和高度重视的特定服务提供的服务。 受过培训的人员，例如中心可以提供的人员。在之前的资助期间，三 提供了核心项目：转基因动物项目、病毒载体项目和基因 表达程序。分子生物学核心新增微阵列基因芯片项目，并已 本质上更新了基因表达计划，以提供这些技术的教育和培训 在接受一定的理论和实践培训后，可以在研究者自己的实验室中进行。 由于研究人员的需求可能已经超出了基本的基因表达方法，涉及此类 染色质结构和分析等技术，这个主要的教育项目已更名为 分子技术培训计划。需要基本分子技术帮助的研究人员， 例如，定量 RT-PCR 和更复杂的技术，例如染色质免疫沉淀测定 （ChIP），可以接受核心主任（Merchant 博士）及其同事（白博士）的培训。