MOLECULAR BIOLOGY CORE

分子生物学核心

• 批准号: 7002129
• 负责人: JUANITA L. MERCHANT
• 金额: $ 15.71万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7002129
• 关键词: biomedical facility; gastrointestinal system; laboratory mouse; laboratory rat; microarray technology; model design /development; molecular biology; science education; transfection /expression vector

The purpose of the Molecular Biology core is to facilitate the application of the tools of molecular genetics to the study of gastrointestinal peptides and their physiological functions. In all aspects, the Molecular Biology Core functions to provide essential services and serves as an educational resource to all Center Investigators. Although basic molecular techniques are widely available to Center investigators through commercial sources, there remain specific services that are best provided by pooled resources and by highly trained personnel such as those that can be provided by a Center. During the prior funding period, three Core programs were provided: the Transgenic Animal Program, the Viral Vector Program and the Gene Expression Program. The Molecular Biology Core has added a Microarray Gene Chip Program and has essentially updated the Gene Expression Program to provide education and training in those techniques that can be performed in the investigators own laboratory after receiving some theoretical and practical training. Since investigator needs have likely moved beyond basic gene expression approaches to involve such techniques as chromatin structure and analysis, this primarily education program has been renamed the Molecular Techniques Training Program. Investigators needing assistance with basic molecular techniques, e.g., quantitative RT-PCR and more sophisticated techniques, e.g., chromatin immunoprecipitation assays (ChIP), can receive training from the Core Director (Dr. Merchant) and her associate (Dr. Bai).

分子生物学核心的目的是促进分子遗传学工具的应用 研究胃肠道肽及其生理功能。在各个方面，分子 生物学核心功能提供基本服务，并作为所有中心的教育资源 调查人员。尽管基本的分子技术可通过 商业资料来源，仍然有特定的服务最好由汇集资源提供，高度提供 受过训练的人员，例如可以由中心提供的人员。在以前的资金期间，三个 提供了核心计划：转基因动物程序，病毒矢量程序和基因 表达程序。分子生物学核心添加了一个微阵列基因芯片程序，并具有 本质上更新了基因表达计划，以提供这些技术的教育和培训 可以在接受一些理论和实用培训后在调查人员自己的实验室中进行。 由于研究者的需求可能已经超越了基本基因表达方法，以涉及这样 技术作为染色质结构和分析，该主要教育计划已重命名 分子技术培训计划。需要对基本分子技术协助的研究人员， 例如，定量RT-PCR和更复杂的技术，例如染色质免疫沉淀测定 （CHIP），可以接受核心董事（商人博士）及其同事（Bai博士）的培训。