Transcriptional and genic basis of incipient speciation

早期物种形成的转录和基因基础

• 批准号: 7245863
• 负责人: CHUNG-I WU
• 金额: $ 33.23万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7245863
• 关键词: Animal Model; Architecture; Automobile Driving; Behavior; Behavioral; Chromosome Mapping; Chromosomes, Human, Pair 3; Classification; DNA Markers; DNA Sequence; Differentiated Gene; Elements; Gene Expression; Genes; Genetic; Genetic Transcription; Genie; Genomics; Genotype; Goals; Head; Light; Link; Literature; Maps; Molecular Profiling; Nucleotides; Numbers; Organ; Partner in relationship; Phenotype; Play; Primates; Quantitative Trait Loci; Race; Recombinants; Resolution; Role; Site; Staging; Transcriptional Regulation; Variant; Zimbabwe; base; gene replacement; genome wide association study; interest; reproductive; sex; trait

DESCRIPTION (provided by applicant): Our general goal is to understand the genetic and transcriptional bases of phenotypic divergence at an early stage of speciation. We shall study the divergence in mating behaviors between the Z (for Zimbabwe) and M (cosmopolitan) behavioral races of Orosophila melanogaster (Wu et al. 1995; Hollocher et al. 1997a, b; Ting et al. 2001). Aim 1 - At the genotypic level, we will identify nucleotide sites that are strongly differentiated between the Z and M races in a whole-genome scan of expressed genes. These race-differentiating sites will anchor genetic mapping of transcription and phenotypic divergence. We will construct multiply recombined (MR) lines for Chromosome 3 between the Z- and M-races for such mapping. Aim 2 - At the transcriptional level, we shall study the expression profiles of the MR lines (in heads and reproductive organs of the 2 sexes) and correlate these profiles with their underlying genotypes. The regulation of transcription is important in linking phenotype and genotype. Aim 3 - At the phenotypic level, we shall first attempt to find the transcriptional basis of the phenotypic divergence (behavioral isolation), using the MR lines. We shall also link phenotypic divergence to genotypic changes directly. We expect to identify a number of candidate "speciation genes,* and will carry out precise gene replacement (Rong and Golic 2000; Greenberg et al. 2003) to confirm their effects on the mating behavior.

描述（由申请人提供）：我们的总体目标是了解物种形成早期阶段表型分化的遗传和转录基础。我们将研究黑腹果蝇 Z（津巴布韦）和 M（世界主义）行为种族之间交配行为的差异（Wu 等人，1995 年；Hollocher 等人，1997a, b；Ting 等人，2001）。目标 1 - 在基因型水平上，我们将在表达基因的全基因组扫描中识别 Z 种族和 M 种族之间强烈差异的核苷酸位点。这些种族分化位点将锚定转录和表型差异的遗传图谱。我们将在 Z 种族和 M 种族之间构建 3 号染色体的多重重组 (MR) 系，以进行此类映射。目标 2 - 在转录水平，我们将研究 MR 系（两性的头部和生殖器官）的表达谱，并将这些谱与它们的潜在基因型相关联。转录调控对于连接表型和基因型很重要。目标 3 - 在表型水平上，我们首先尝试使用 MR 系找到表型分歧（行为隔离）的转录基础。我们还将表型分歧与基因型变化直接联系起来。我们期望鉴定出一些候选“物种形成基因”*，并将进行精确的基因替换（Rong and Golic 2000；Greenberg et al. 2003）以确认它们对交配行为的影响。