ASI-Spectral Karyotyping (SKY) System

ASI-光谱核型分析 (SKY) 系统

• 批准号: 6877535
• 负责人: MURIEL T DAVISSON
• 金额: $ 18.5万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877535
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; colorimetry; cytogenetics; karyotype; spectrometry

DESCRIPTION (provided by applicant): New genetic technologies combined with a well-characterized genome make the mouse the preferred mammalian model for understanding human diseases and identifying disease genes. Investigators must be able to characterize these models both physiologically and genetically to exploit their full biomedical potential. To realize this potential investigators must make the same basic clinical assessments used in human diagnostic medicine, often including chromosomal and histological analyses. The staff of The Jackson Laboratory (TJL) is at the forefront both in defining the normative biology of standard inbred mice and in characterizing hundreds of new spontaneous and induced mutations of biomedical significance; karyotypic analysis is an essential aspect of this characterization. TJL investigators currently rely on limited part-time cytogenetic services provided by Dr. Davisson's research personnel through her cytogenetic research program. This compromises both the productivity of her program and the research progress in programs requiring karyotypic analysis. Funding is requested for the purchase of a semi-automated spectral karyotyping system, the ASI-SKY System, Applied Spectral Imaging, Inc. Vista CA, that has proven its reliability in human diagnostic medicine and in basic research settings. This system, which also delivers quantitative spectral tissue analysis, represents a cost-efficient mechanism for cytogenetic assessment without requiring cytogenetic expertise, thus extending such analytical capabilities to all interested investigators. The ASI-SKY System will directly enhance the work of 17 TJL investigators with 34 NIH-funded grants who currently lack access to semi-automated, quantitative, digital spectral imaging instrumentation. The ASI-SKY system offers 4 capabilities unavailable at TJL: 1) semi-automated mouse chromosome karyotyping, 2) quantitative measurement of tissue-specific differences in mutant and control mice, 3) simultaneous spectroscopic assessment of multiple distinct proteins in the same tissue sample and 4) capture, analysis and simple colorimetric presentation of emission spectra from every pixel in an image. Delivery of technician-assisted, fee-for-service access to this state-of-the-art instrumentation by the Biological Imaging Service under the guidance of Dr. Davisson, an established cytogeneticist, will greatly enhance ongoing research efforts and assure maximum use of such instrumentation, thus providing a cost-effective way to fill an urgent, and currently unmet need of TJL Research Staff.

描述（由申请人提供）：新的遗传技术与充分表征的基因组相结合，使小鼠成为了解人类疾病和识别疾病基因的首选哺乳动物模型。研究人员必须能够从生理学和遗传学上表征这些模型，以充分发挥其生物医学潜力。为了实现这一点，潜在的研究人员必须进行与人类诊断医学相同的基本临床评估，通常包括染色体和组织学分析。杰克逊实验室 (TJL) 的工作人员在定义标准近交小鼠的规范生物学以及表征数百个具有生物医学意义的新自发和诱导突变方面处于前沿；核型分析是该表征的一个重要方面。 TJL 研究人员目前依赖 Davisson 博士的研究人员通过她的细胞遗传学研究项目提供的有限兼职细胞遗传学服务。这既影响了她的项目的生产力，也影响了需要核型分析的项目的研究进展。申请资金用于购买半自动光谱核型分析系统 ASI-SKY 系统（Applied Spectral Imaging, Inc. Vista CA），该系统已在人类诊断医学和基础研究环境中证明了其可靠性。该系统还提供定量光谱组织分析，代表了一种经济有效的细胞遗传学评估机制，无需细胞遗传学专业知识，从而将这种分析能力扩展到所有感兴趣的研究人员。 ASI-SKY 系统将直接加强 17 名 TJL 研究人员的工作，这些研究人员获得了 34 项 NIH 资助，他们目前无法使用半自动、定量、数字光谱成像仪器。 ASI-SKY 系统提供 TJL 不具备的 4 种功能：1) 半自动小鼠染色体核型分析，2) 突变体和对照小鼠组织特异性差异的定量测量，3) 对同一组织样本中多种不同蛋白质的同时光谱评估4) 捕获、分析和简单比色显示图像中每个像素的发射光谱。在资深细胞遗传学家 Davisson 博士的指导下，生物成像服务中心提供技术人员协助、按服务收费的方式使用这种最先进的仪器，这将极大地加强正在进行的研究工作，并确保最大限度地利用此类仪器，从而提供了一种具有成本效益的方式来满足 TJL 研究人员目前未满足的迫切需求。