TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7391976
• 负责人: MURIEL T DAVISSON
• 金额: $ 27.08万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391976
• 关键词: TRANSGENIC; TARGETED; MUTANT; MICE; PRESERVATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS This competing renewal seeks continued funding for The Jackson Laboratory''''''''s (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。关于援助，这种竞争的更新寻求继续为杰克逊实验室'''''''S（TJL）诱导的突变资源（IMR）继续提供资金，以继续向科学界提供转基因和靶向突变小鼠的关键资源。通过基因转移（转基因小鼠），同源重组以及时间和组织特异性产生以及通过这些手段产生的诱导的突变小鼠的使用有选择地改变小鼠基因组，这已经迅速扩展，增加了维持，保存，保存和分配这些突变小鼠的资源的需求，并在高健康状态和最小的限制下分配了这些突变小鼠。研究人员经常收到他们创建的小鼠的要求，并希望相信将小鼠分配给其他科学家在研究计划和赠款中的负担。 TJL成立于1929年，是一家非营利性研究机构，已将小鼠的突变菌株维持和分布近50年。 1992年，TJL建立了IMR，其目的是进口，冷冻水果和分布生物医学上重要的诱导突变小鼠。对IMR的需求是令人信服的，并通过其快速增长来证明。自从开始以来，IMR接受了495个突变股，其中299个分发，目前还有58个正在进口过程中。 1997年，分发了近60,000只小鼠。我们要求支持重新培养，冷冻保存和应变发展以及应变发育，以提高IMR菌株的科学价值；将小鼠维持在尽可能多的成本恢复基础上向其他科学家分发。为了改善IMR资源计划，我们建议进行研究：（1）开发方法来确保小鼠精子的无病原体细胞透露率保存，（2）表型在表型上表征了新的诱导的突变和转移到新的良性遗传背景的突变，以及（3）开发出更具成本效益的基因分型方法。对IMR小鼠的需求不断增加，随着几个不可再生的启动赠款终止，资金的下降是一致的。被拒绝菌株的比例正在增加（请参阅简介）。需求的大小超出了NCRR的资金范围。 Niaid和Niams已经通过机构内部协议为这一努力做出了贡献。在当前赠款的前4年中，IMR提供的小鼠中有70％是由16个NIH研究所的NIH壁内和外部授予者收到的。为了满足日益增长的需求，我们要求批准扩展这种转基因和有针对性小鼠的国家资源，以授权NCRR向NIH Institutes寻求额外的资金。为了帮助审查，我们已将整体预算组件细分。引言解释了当前的资金机制以及我们扩大IMR的建议，而不会承担NCRR的负担。