UAB Neuroscience Core Center

阿拉巴马大学神经科学核心中心

• 批准号: 7089129
• 负责人: John J. Hablitz
• 金额: $ 46.86万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7089129
• 关键词: biomedical facility; neurosciences

DESCRIPTION (provided by applicant): The UAB Neuroscience Core Center will provide core support services for a highly productive group of 18 NINDS funded investigators and their neuroscience research activities. The proposed cores are designed in response to investigators' inputs and will promote better science, reduce costs to individual investigators and increase productivity. The goal is to enhance the research environment by providing high quality research core support services within a scientifically stimulating multidisciplinary setting. The Center will consist of four cores. Core A is a Behavioral Assessment Core which will provide a facility for the testing of transgenic mice using the most accepted battery of behavioral, including a primary behavioral screen, sensory and motor tests, an open field test for emotionality and exploratory activity and cognitive testing. The Core will also assist investigators in the development of tools that are needed by them for a more detailed assessment of specific behaviors. Core B is a Molecular Detection Core (MDC) to meet an unmet need in the UAB neuroscience community for sensitive, automated immunochemistry (IHC) and in situ hybridization (ISH) detection. The MDC will offer both chromogenic and fluorescent detection options as well as perform multi-labeling IHC and ISH procedures. Core C is a Protein Interaction Core to provide (1) expression cloning of cDNAs and genomic sequences in prokaryotic and eukaryotic vectors for characterization of protein interactions in in vitro systems and in viable cells; (2) two-hybrid screening using mammalian, yeast and bacterial system to identify interactions between proteins and (3) establish a web based information sharing resource that will provide a comprehensive list of vectors, expression and screening systems, and relevant applications by NINDS investigators at UAB and elsewhere. Core D is an Administrative Core which will provide continuous, high quality administrative and communication support for the individual Core Directors and the UAB Neuroscience Core Center investigators. Core D will create and maintain a website to facilitate communication and sharing for resources and methodologies. All Cores provide novel, state of the art facilities that are not currently available to UAB NINDS-supported investigators.

描述（由申请人提供）： UAB神经科学核心中心将为由Ninds资助的18个研究人员及其神经科学研究活动组成的高效群体提供核心支持服务。拟议的核心是为了响应研究人员的投入而设计的，并将促进更好的科学，降低个人研究人员的成本并提高生产率。目标是通过在科学刺激的多学科环境中提供高质量的研究核心支持服务来增强研究环境。该中心将由四个核心组成。核心A是一种行为评估核心，它将使用最接受的行为范围来为转基因小鼠进行测试，包括主要的行为屏幕，感觉和运动测试，进行情绪和探索性活动和认知测试的开放式现场测试。核心还将协助研究人员开发他们所需的工具，以更详细地评估特定行为。核心B是一种分子检测核心（MDC），可满足UAB神经科学界未满足的敏感，自动免疫化学（IHC）和原位杂交（ISH）检测的需求。 MDC将提供发色和荧光检测选项，以及执行多标签的IHC和ISH程序。核C是一种蛋白质相互作用核心，可（1）在原核生物和真核载体中cDNA和基因组序列的表达克隆，用于表征体外系统和活细胞中蛋白质相互作用的表征； （2）使用哺乳动物，酵母和细菌系统进行两次杂交筛查，以识别蛋白质之间的相互作用，（3）建立基于Web的信息共享资源，该资源将提供媒介，表达和筛选系统的全面列表，以及NINDS研究人员的相关应用在UAB和其他地方。核心D是一种行政核心，将为单个核心主管和UAB Neuroscience Core Center中心调查人员提供持续，高质量的行政和沟通支持。核心D将创建和维护网站，以促进与资源和方法共享的沟通和共享。所有核心都提供新颖的，最先进的设施，目前尚不适用于UAB Ninds支持的研究人员。