UAB Neuroscience Core Center

阿拉巴马大学神经科学核心中心

基本信息

批准号：
9192831
负责人：
John J. Hablitz
金额：
$ 58.8万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-07-01 至 2020-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9192831
关键词：
Advanced Development Alzheimer&apos s Disease Area Asses Behavior Behavior assessment Behavioral Biochemical Budgets CRISPR/Cas technology Clustered Regularly Interspaced Short Palindromic Repeats Cognition Collaborations Communities Complement Complementary DNA Computer software Consultations Core Facility Detection Development Effectiveness Electroencephalography Elements Environment Epilepsy Fostering Functional disorder Funding Genes Genetic Home environment Human Resources Image Analysis Immunohistochemistry In Situ Hybridization Individual Institution Interdisciplinary Study Investments Label Laboratories Leadership Light Mediating Mental disorders Methods Mission Molecular Molecular Genetics Mus National Institute of Neurological Disorders and Stroke Nerve Degeneration Nervous System Physiology Neurosciences Neurosciences Research Personnel Staffing Phenotype Prevention Procedures Productivity Progress Reports Proteins Qualifying Quantum Dots Rattus Recombinant Proteins Research Research Activity Research Design Research Infrastructure Research Personnel Research Project Grants Resources Rodent Science Scientist Services Simplexvirus Site Source Specimen Stress Subfamily lentivirinae Talents Technical Expertise Techniques Technology Testing Training Viral Packaging Virus awake behavior test behavioral study cognitive testing cost effective cost efficient design expression cloning graduate student innovation interest investigator training meetings nervous system disorder neurodevelopment novel optogenetics programs protein expression protein purification protocol development research facility tool vector virus core

项目摘要

This application seeks continued support for the UAB Neuroscience Core Center which is currently entering its' tenth year of providing service and training to the UAB neuroscience community. This Center will add innovative new core research facilities while maintaining the productive resources present in existing cores. The principal focus of the UAB Neuroscience Core Center will be to facilitate cost-effective, innovative and interdisciplinary neuroscience research in areas pertinent to the NINDS mission. Genetic, molecular, cellular and behavioral approaches are used by a group of 17 NINDS supported scientists with a total of 19 R01 projects. The qualifying projects have increasingly relied on shared core facilities and the collaborative UAB scientific environment to accomplish their research objectives. The proposed Cores complement each other and provide advanced technical expertise that will result in exciting new collaborations. The Behavioral Assessment Core (Core A) provides both service-oriented behavioral phenotyping and research staff training to investigators using rodents. New facilities for optogenetic stimulation during behavioral/cognitive testing will be added along with semi-automated home-cage testing. The Molecular Detection and Stereology Core (Core B) will continue to offer sensitive, standardized immunohistochemistry and in situ hybridization technologies. The utility of this core will be enhanced adding facilities for quantitative stereology. Focus on protocol development and investigator training will transfer these methods to individual investigators The Vector and Virus Core (Core C) will accelerate genetic and biochemical studies of NINDS investigators by providing lentiviral and AAV viruses for optogenetic probes, provide services for CRISPR/Cas 9 mediated gene editing and continue to provide services for protein interaction studies. Although there are commercial sources for some of the services proposed, it should be stressed that an important aspect of the UAB Cores is that, being on site, consultation is continually available and accessible even after the service is provided. The Administrative Core (Core D) will provide administrative and scientific leadership. Collectively, the Cores promote multidisciplinary collaboration, exchange of information and technological advances. The Core Center attracts new investigators to neuroscience research, develop collaborative research activities with other institutions and increase the quality and productivity of funded research projects in a cost-effective manner

该申请寻求对 UAB 神经科学核心中心的持续支持，该中心目前正在进入为 UAB 神经科学界提供服务和培训的第十个年头。这中心将在保持生产资源的同时增加创新的核心研究设施存在于现有核心中。 UAB 神经科学核心中心的主要重点是促进相关领域的具有成本效益、创新和跨学科的神经科学研究 NINDS 使命。一组使用遗传、分子、细胞和行为方法 17 NINDS 为科学家提供了总计 19 个 R01 项目的支持。符合条件的项目有越来越依赖共享核心设施和协作 UAB 科学环境完成他们的研究目标。拟议的核心相互补充并提供先进的技术专业知识将带来令人兴奋的新合作。行为方面评估核心（核心 A）提供面向服务的行为表型分析和研究人员使用啮齿动物对调查人员进行培训。光遗传学刺激的新设施行为/认知测试将与半自动笼式测试一起添加。这分子检测和体视学核心（核心 B）将继续提供灵敏、标准化的免疫组织化学和原位杂交技术。该核心的实用性将会增强添加定量体视学设施。专注于方案开发和研究者培训将把这些方法转移给个别研究人员载体和病毒核心（核心 C）将通过提供慢病毒和 AAV 加速 NINDS 研究人员的遗传和生化研究用于光遗传学探针的病毒，为 CRISPR/Cas 9 介导的基因编辑提供服务继续为蛋白质相互作用研究提供服务。尽管有商业来源在提议的一些服务中，应该强调的是 UAB 核心的一个重要方面是即使在服务结束后，在现场也可以持续进行咨询假如。行政核心（核心 D）将提供行政和科学领导。总的来说，核心促进多学科合作、信息交流和技术进步。核心中心吸引新的研究人员进行神经科学研究，与其他机构开展合作研究活动，提高质量和以具有成本效益的方式提高受资助研究项目的生产力