Synthetic and Analytical Chemistry

合成与分析化学

• 批准号: 7190573
• 负责人: CHARLES R IDEN
• 金额: $ 19.03万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7190573
• 关键词: DNA damage; biomedical facility; chemical synthesis; electrophoresis; high performance liquid chromatography; mass spectrometry; method development; nucleosides; nucleotides

The Synthetic and Analytical Core Project forms the underpinning for all research projects in the Program by furnishing modified oligodeoxynucleotides and analytical services for all investigators. The objectives of the Core are (a) to synthesize modified deoxynucleosides that represent endogenous DNA damage and to convert them to the 5'-O-DMT-3'-O-phosphoramidites for incorporation into oligodeoxynucleotides; (b) to generate highly pure, site-specifically modified oligodeoxynucleotides using automated DNA synthesis techniques; and (c) to provide essential analytical services, including mass spectrometry, HPLC, and capillary electrophoresis, for all participants in the program. In addition, the Core Facility will develop new synthetic methods and analytical techniques needed for Program research and for the operation of a state-of-the-art facility. Combining the synthetic and analytical aspects of Program research in a Core facility operated with highly trained personnel will guarantee timely access to modified oligodeoxynucleotides for all Program participants and ensure that major equipment items are available and functioning.

合成和分析核心项目通过为所有研究人员提供修饰的寡脱氧核苷酸和分析服务，构成了该计划中所有研究项目的基础。核心的目标是 (a) 合成代表内源性 DNA 损伤的修饰脱氧核苷，并将其转化为 5'-O-DMT-3'-O-亚磷酰胺，以掺入寡脱氧核苷酸中； (b) 至 使用自动化 DNA 合成技术生成高纯度、位点特异性修饰的寡脱氧核苷酸； (c) 为该计划的所有参与者提供必要的分析服务，包括质谱、高效液相色谱和毛细管电泳。此外，核心设施将开发项目研究和最先进设施运营所需的新合成方法和分析技术。将计划研究的合成和分析方面结合在由训练有素的人员操作的核心设施中，将保证所有计划参与者及时获得修饰的寡脱氧核苷酸，并确保主要设备项目可用且正常运行。