Macromolecular X-ray Structure Facility Instrumentation

高分子X射线结构设备仪器

• 批准号: 6578685
• 负责人: ANDREW D MESECAR
• 金额: $ 34.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6578685
• 关键词: X ray crystallography; biomedical equipment purchase; data collection methodology /evaluation; macromolecule; monitoring device; structural biology

DESCRIPTION (provided by applicant): A state-of-the-art imaging plate area detector with variable 20 stage, low-temperature cooling apparatus, and confocal multilayer optics will be purchased to replace older instrumentation. It will be used to collect x-ray diffraction data from single crystals on a rotating-anode x-ray generator equipped with a Cu target. The unit will be used to expand and enhance the capabilities of the saturated multi-user, macromolecular, single crystal, x-ray diffraction facility in the Molecular Biology Research Building (MBRB) at the University of Illinois at Chicago (UIC). This facility, the UIC/RRC (Research Resources Center) Macromolecular Structure Facility (MSF), is located on the west medical campus, and currently supports seven (Gettins, Jeffery, Johnson, Lavie, Matsumura, Mesecar, Volz) major research groups, including two NIH program grants and one NIH center. Approximately 80% of the instrument time will be utilized by NIH-funded researchers, and the remaining time will be available for other researchers on the UIC campus, in the Chicago area, and for a collaborator in North Carolina. The present research facility was established four years ago, and is used to augment courses in Medicinal Chemistry, Pharmacognosy, Microbiology, Biochemistry, and Pharmaceutical Biotechnology. Access to the instrumentation by new users both on and off campus has been governed by an administrative oversight and advisory committee including campus researchers and RRC personnel. The two area detectors and optics now in operation are heavily used, and are somewhat dated and aging, resulting in substantial downtime and increased maintenance. The Siemens multi-wire area detector, that will be replaced, has no low-temperature cooling system, and is therefore unsuitable for cryo-stored or unstable samples. The new detector and state-of-the-art optics system combination will allow for faster and more accurate in-house data collection on both routine and difficult samples including those with long cell axes, small crystal size, or poor diffraction, as well as provide for faster and more thorough screening of crystals in preparation for synchrotron time. The increased use of the UIC/RRC MSF facility reflects the growing importance of structural biology as a major component in research and teaching at the University of Illiniois at Chicago. The growth in structural biology research at UIC is being fostered by the University Administration and the structural biology community, and is reflected in the establishment of the Center for Pharmaceutical Biotechnology, and most recently the establishment of the Center for Structural Biology.

描述（由申请人提供）： 将购买最先进的成像板区域探测器，配备 20 个可变级、低温冷却装置和共焦多层光学器件，以取代旧仪器。它将用于在配备铜靶的旋转阳极 X 射线发生器上收集单晶的 X 射线衍射数据。该装置将用于扩展和增强伊利诺伊大学芝加哥分校 (UIC) 分子生物学研究大楼 (MBRB) 饱和多用户、大分子、单晶 X 射线衍射设施的功能。该设施，UIC/RRC（研究资源中心）大分子结构设施（MSF），位于西医学园区，目前支持七个主要研究小组（Gettins、Jeffery、Johnson、Lavie、Matsumura、Mesecar、Volz），包括两项 NIH 项目拨款和 1 个 NIH 中心。大约 80% 的仪器时间将由 NIH 资助的研究人员使用，剩余时间将供伊利诺伊大学芝加哥分校校园、芝加哥地区的其他研究人员以及北卡罗来纳州的合作者使用。目前的研究设施成立于四年前，用于补充药物化学、生药学、微生物学、生物化学和制药生物技术等课程。校园内外新用户对仪器的访问由包括校园研究人员和 RRC 人员在内的行政监督和咨询委员会管理。目前正在运行的两个区域探测器和光学器件被大量使用，并且有些过时和老化，导致大量的停机时间和增加的维护。将要更换的西门子多线区域检测器没有低温冷却系统，因此不适合低温储存或不稳定的样品。新的探测器和最先进的光学系统组合将允许对常规样品和困难样品进行更快、更准确的内部数据收集，包括长细胞轴、小晶体尺寸或较差衍射的样品，以及提供更快、更彻底的晶体筛选，为同步加速器时间做好准备。 UIC/RRC MSF 设施的使用增加反映了结构生物学作为伊利诺伊大学芝加哥分校研究和教学的主要组成部分的重要性日益增加。 UIC 结构生物学研究的发展得到了大学管理部门和结构生物学界的大力支持，这体现在药物生物技术中心的建立以及最近结构生物学中心的建立上。

项目成果

Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.

结核分枝杆菌四聚体 IIa 类果糖 1,6-二磷酸醛缩酶催化的结构基础。

• 发表时间: 2009-03-06
• 影响因子: 5.6
• 作者: Pegan, Scott D;Rukseree, Kamolchanok;Franzblau, Scott G;Mesecar, Andrew D
• 通讯作者: Mesecar, Andrew D

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.

通过对来自嗜热脂肪地芽孢杆菌的高度热稳定性磷酸三酯酶样内酯酶的鉴定和表征揭示了热稳定性的结构基础。

• 发表时间: 2009-08-15
• 影响因子: 3.9
• 作者: Hawwa, Renda;Aikens, John;Turner, Robert J;Santarsiero, Bernard D;Mesecar, Andrew D
• 通讯作者: Mesecar, Andrew D

Evaluating the 3C-like protease activity of SARS-Coronavirus: recommendations for standardized assays for drug discovery.

评估 SARS 冠状病毒的 3C 样蛋白酶活性：药物发现标准化测定的建议。

• 发表时间: 2008-04
• 影响因子: 5
• 作者: Grum;Ratia, Kiira;Begaye, Adrian;Baker, Susan C;Mesecar, Andrew D
• 通讯作者: Mesecar, Andrew D

Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.

炭疽杆菌两种谷氨酸消旋酶同工酶的结构和功能分析及其对抑制剂设计的影响。

• 发表时间: 2007-08-31
• 影响因子: 5.6
• 作者: May, Melissa;Mehboob, Shahila;Mulhearn, Debbie C;Wang, Zhiqiang;Yu, Huidong;Thatcher, Gregory R J;Santarsiero, Bernard D;Johnson, Michael E;Mesecar, Andrew D
• 通讯作者: Mesecar, Andrew D