Laser Capture Microdissection System

激光捕获显微切割系统

• 批准号: 6876004
• 负责人: JOHN B SCHWEITZER
• 金额: $ 21.05万
• 依托单位: EAST TENNESSEE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876004
• 关键词: Chlamydia trachomatis; biomedical automation; biomedical equipment purchase; biotechnology; cell population study; diagnostic tests; heart disorder diagnosis; laser capture microdissection; myocardial ischemia /hypoxia; reproductive system disorder diagnosis; reproductive system infection; single cell analysis; toll like receptor

DESCRIPTION (provided by applicant): This request is for an Arcturus AutoPix II, which is an automated laser capture microdissection microscope equipped for both brightfield and fluorescent operation. The system will significantly enhance the research of 4 major users who are conducting NIH-funded R01 research projects and 2 minor users, one of whom holds an R21. Drs. Wyrick and Raulston would use LCM technology to analyze tissue subpopulations of genital epithelial cells that are susceptible to infection by Chlamydia trachomatis. Such samples would be examined for transcription and/or protein expression of (i) estrogen receptors involved in Chlamydia attachment, (ii) chemokines produced by infected cells, and (iii) Chlamydial iron-responsive genes and proteins; uninfected, non-susceptible genital cells within the same tissue would serve as comparative controls. Dr. Hoover will use the instrument to isolate contents of single or multiple neurons of guinea pig intracardiac ganglia and use RT PCR to determine the presence of mRNA coding for tachykinin receptors and receptors for calcitonin gene-related peptide and related mediators. Dr. Li will employ this equipment to examine the role of Toll-like receptors (TLRs) in myocardial ischemia/reperfusion injury. In ischemic and non-ischemic tissue captured through the microdissection, protein expression and transcription for TLRs, selected cytokines and for various protein kinases will be assayed. The Arcturus instrument is the standard instrument of its kind. The East Tennessee State University College of Medicine has a strong institutional commitment to the acquisition and maintenance of this microscope system. A highly experienced and full time research assistant, Johanna Preiszner, Ph.D., will oversee the day to day operation of the microscope. A room of about 100 square feet in the Department of Pathology will house the system. The College of Medicine has also agreed to underwrite the service contract for the instrument after the initial warranty year.

描述（由申请人提供）：此请求针对 Arcturus AutoPix II，它是一种自动激光捕获显微解剖显微镜，配备明场和荧光操作。该系统将显着增强正在进行 NIH 资助的 R01 研究项目的 4 个主要用户和 2 个次要用户（其中​​一名持有 R21）的研究。博士。 Wyrick 和 Raulston 将使用 LCM 技术来分析易受沙眼衣原体感染的生殖器上皮细胞的组织亚群。将检查这些样本的转录和/或蛋白质表达：（i）参与衣原体附着的雌激素受体，（ii）受感染细胞产生的趋化因子，以及（iii）衣原体铁反应基因和蛋白质；同一组织内未感染、不敏感的生殖细胞将作为比较对照。 Hoover博士将使用该仪器分离豚鼠心内神经节的单个或多个神经元的内容物，并使用RT PCR来确定编码速激肽受体和降钙素基因相关肽受体及相关介质的mRNA的存在。李博士将利用该设备来研究Toll样受体（TLR）在心肌缺血/再灌注损伤中的作用。在通过显微切割捕获的缺血和非缺血组织中，将分析 TLR、选定的细胞因子和各种蛋白激酶的蛋白质表达和转录。 Arcturus 仪器是同类仪器中的标准仪器。东田纳西州立大学医学院对购买和维护该显微镜系统做出了强有力的机构承诺。经验丰富的全职研究助理 Johanna Preiszner 博士将负责监督显微镜的日常操作。病理科的一个约 100 平方英尺的房间将容纳该系统。医学院还同意在初始保修年后承保该仪器的服务合同。