NEUROPATHOLOGY AND BRAIN BANK CORE

神经病理学和脑库核心

• 批准号: 6808098
• 负责人: Daniel P Perl
• 金额: $ 21.59万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6808098
• 关键词: Alzheimer' s disease; biomedical facility; brain; brain disorder diagnosis; clinical research; genetically modified animals; human tissue; laboratory mouse; neuroanatomy; neuropathology; postmortem; tissue /cell preparation; tissue /organ preservation; tissue resource /registry

The Neuropathology Core will continue to play a vital role in the ongoing Program. The Neuropathology Core serves to obtain autopsy-derived brain specimens from individuals who have been evaluated and followed longitudinally by the Clinical Core especially residents of the Jewish Home and Hospital for the Aged, a very large academically affiliated nursing home. We strive to obtain the brain specimens with the shortest post-mortem interval and for the entire specimen collection the mean postmortem interval is less than 6 hours. The specimens are dissected and preserved in a manner that maximizes their utility for the needs of both the proposed experiments within the Program as well as other research projects within the Mount Sinai ADRC as well as independent aging-related projects. This includes snap freezing one half of the brain specimen and fixing the other half is freshly prepared paraformaldehyde. The dissection protocol in place allows for the preparation of selected regions of the brain in such a way that the non-biased sampling techniques of stereology can be applied to quantify lesion and neuron numbers. A detailed neuropathologic workup is carried out to establish a neuropathologic diagnosis as well as to document the extent and distribution of relevant neuropathologic lesions. These data are entered into an extensive data base which can be integrated into the clinical data base for the purpose cliniconeuropathologic correlative investigations. The Neuropathology Core will also provide assistance and expertise in the morphologic evaluation of newly developed transgenic animals as well as those transgenic animals exposed to experimental manipulations. We will interpret the nature of any identified lesions for their relevance as models of AD or other human neurodegenerative conditions. Because this Brain Bank has been operating for approximately 18 years, an efficient and effective operating structure for the Brain Bank already exists. The tissues we have collected have been extensively used in a wide range of studies. They are extensively requested both by researchers within the ADRC, the greater Mount Sinai research community and by many other investigators throughout the US and even internationally. The overall aim of this core is to continue to maintain and operate the Brain Bank in such a way as to meet the needs of the cores and studies in the ADRC in an optimal fashion.

神经病理学核心将继续在正在进行的计划中发挥至关重要的作用。神经病理学核心有助于从经过评估和纵向纵向的人的个人中获得尸检衍生的大脑标本，尤其是在犹太人家庭和医院的居民中，是一个年龄较大的学术界隶属养老院。我们努力以最短的验尸间隔获得大脑样本，对于整个样品收集，平均验尸间隔小于6小时。剖析和保存样品，以最大化计划中提议的实验以及其他研究项目的需求最大化其实用性 西奈山ADRC以及与衰老相关的独立项目。这包括将一半的脑标本冻结并固定另一半是新鲜准备的多聚甲醛。解剖方案允许制备大脑选定区域的方式，使得可以将无偏见的立体抽样技术应用于量化病变和神经元数量。进行了详细的神经病理检查，以建立神经病理学诊断，并记录相关神经病变病变的程度和分布。这些数据被输入一个广泛的数据库，可以将其集成到临床数据库中，以进行目的 调查。神经病理学核心还将在新开发的转基因动物以及暴露于实验性操纵的那些转基因动物的形态评估方面提供帮助和专业知识。我们将把任何已鉴定的病变的性质解释为其与AD或其他人类神经退行性疾病的模型相关性。因为该大脑银行已经运行了大约18年，所以已经存在了脑库的高效工作结构。我们收集的组织已在广泛的研究中广泛使用。 ADRC中的研究人员，大西奈山研究社区以及许多人都广泛要求它们 全美甚至国际上的其他调查人员。该核心的总体目的是继续以最佳方式满足ADRC的核心和研究的需求，以继续维护和运营大脑库。