CORE--NEUROPATHOLOGY AND BRAIN BANK

核心——神经病理学和脑库

• 批准号: 6299227
• 负责人: Daniel P Perl
• 金额: $ 21.2万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299227
• 关键词: Alzheimer's disease; biomedical facility; brain; brain disorder diagnosis; human tissue; neuropathology; postmortem; tissue resource /registry

The specific aims of the Brain Bank Core are to obtain, characterize, describe, dissect, preserve and distribute to different researchers engaged in Alzheimer's disease research, brain tissues obtained from normal control, and Alzheimer's disease subjects. The Brain Bank Core will obtain and bank brain tissues from as many of the subjects enrolled by the Recruitment and Diagnosis Core and studied by the Neurophyschology project as possible. The Brain Bank Core will perform autopsies with the lease possible post-mortem delay, and will maintain permanently all pertinent specimen related information in a manner accessible to all past, present and future investigators using the Brain Bank. The acquisition and banking of the projected cohorts will provide a unique resource to only for the proposed studies, but for generations of studies and investigators to come. The brain banking procedures outlined in this application have been designed to preserved the tissues optimally for as broad a set of experimental methodologies as possible. After extraction, brain specimens will be photographed, weighted and any gross lesions and abnormalities note. The right half of the brain will be preserved in paraformaldehyde for neuropathological and neuroanatomical studies. The left half will be dissected in 0.5-0.8 cm coronal slabs, and after identification of the structures in each slab they will be snap frozen in liquid freon. Frozen slabs will be kept at -80C until sub-dissected for use by specific studies. The Brain Bank Core will serve all five projects proposed in this application either directly or indirectly. The Brain Bank Core will contribute to the Neuropsychology, Family History and Acute Phase Reactants projects (Projects 1, 2 and 4) by performing autopsies of subjects who have been studied in life. The neuropathological information obtained (Project 5) on these cases will enhance the Neuropsychology project by providing data on the neuropathological of cognitive decline in the aged. The Neurochemistry project (Project 3) will use the tissues banked by this core to investigate the neurochemical correlates of cognitive decline in the aged. The procedures used by the Brain Bank Core have been optimized to enhance and facilitate multiple neurochemical studies of each case banked. In addition the acquisition and banking of brain tissues from this unique cohort of subjects will augment the utility of the Brain Bank for projects funded by other grants (eg., ADRC, Cellular and Molecular Markers in Schizophrenia-PPG), by providing specimens from normal elderly controls whose cognitive status has been confirmed by rigorous ante- mortem testing.

脑库核心的具体目标是获取、表征、 描述、剖析、保存并分发给不同的研究人员 从事阿尔茨海默病研究，脑组织取自 正常对照和阿尔茨海默病受试者。 脑库核心 将从尽​​可能多的登记受试者中获取并储存脑组织 由招募和诊断核心进行研究并由神经生理学进行研究 项目尽可能。 脑库核心将与 租用可能的事后延迟，并将永久保留所有 以所有人都可以访问的方式提供相关样本相关信息 过去、现在和未来的研究人员使用脑库。 这 预计群体的收购和银行业务将提供独特的 资源仅用于拟议的研究，但适用于几代研究 以及即将到来的调查人员。 本文概述的脑库程序 应用程序的设计目的是为了最佳地保存组织 尽可能广泛地建立一套实验方法。 提取后， 脑标本将被拍照、称重以及任何肉眼可见的病变 异常注意。 右半脑将被保存在 多聚甲醛用于神经病理学和神经解剖学研究。 这 左半部分将被解剖成0.5-0.8厘米的冠状板，然后 识别每个板中的结构，它们将被快速冻结 在液态氟利昂中。 冷冻板将保存在 -80C 直至进行细分 供具体研究使用。 大脑银行核心将为所有五个人服务 本申请中直接或间接提出的项目。 这 脑库核心将为神经心理学、家族史做出贡献 和急性相反应物项目（项目 1、2 和 4） 对生活中被研究过的对象进行尸检。 这 获得的关于这些病例的神经病理学信息（项目 5）将 通过提供有关以下方面的数据来加强神经心理学项目 老年人认知能力下降的神经病理学。 神经化学 项目（项目 3）将使用该核心储存的组织来 研究认知能力下降的神经化学相关性 年纪大了。 脑库核心使用的程序已经过优化 加强和促进每个病例的多项神经化学研究 存入银行。 此外，还从以下来源获取和储存脑组织： 这个独特的受试者群体将增强脑库的实用性 对于由其他赠款资助的项目（例如 ADRC、细胞和分子 精神分裂症-PPG中的标记），通过提供正常老年人的标本 其认知状态已通过严格的预先确认的对照 尸检测试。