CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6593370
• 负责人: Daniel P Perl
• 金额: $ 19.62万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6593370
• 关键词: Alzheimer's disease; animal tissue; biomedical facility; brain disorder diagnosis; histopathology; human tissue; neuritic plaques; neurofibrillary tangles; neuropathology; postmortem; tissue resource /registry

The Neuropathology Core will continue to play a vital role in the Mount Sinai/Bronx VA Medical Center Alzheimer's Disease Research Center (ADRC). The Neuropathology Core serves to obtain autopsy-derived brain specimens from cases of Alzheimer's disease (AD) who have been evaluated and followed longitudinally by the Clinical Core as well as AD patients identified in the Jewish Home and Hospital for the Aged, a very large academically affiliated nursing home. We strive to obtain the brain specimens will the shortest post-mortem interval and for the entire ADRC specimen collection 39% are obtained within 4 hours and 69% within 6 hours. The specimens are dissected and preserved in a manner that maximizes their utility for the needs of both the proposed experiments within the ADRC as well as other anticipated research projects. This includes snap freezing one half of the brain specimen and fixing the other half is freshly prepared paraformaldehyde. A new addition to the dissection protocol are approaches that allow for the preparation of selected regions of the brain in such a way that the non-biased sampling techniques for stereology can be applied to quantify lesions and neuron numbers. A detailed neuropathology workup is carried out to establish a neuropathologic diagnosis as well as to document the extent and distribution of relevant neuropathologic lesions. These data are entered into an extensive data base which can be integrated into the clinical data base for the purpose cliniconeuropathologic correlative investigations. The Neuropathology Core will also provide assistance and expertise in the morphologic evaluation of newly developed transgenic animals and interpret the nature of any identified lesions for their relevance as models of AD or other human neurodegenerative conditions. Because the ADRC Brain Bank has been operating for approximately 13 years, an efficient and effective operating structure for the Brain Bank already exists. The tissues we have collected have been extensively used in a wide range of studies. They are extensively requested both by researchers within the ADRC, the greater Mount Sinai research community and by many other investigators through the US and even internationally. The overall aim of this core is to continue to maintain and operate the Brain Bank in such a way as to meet the needs of the cores and studies in the ADRC in an optimal fashion.

神经病理学核心将继续在山上发挥至关重要的作用 西奈/布朗克斯VA医疗中心阿尔茨海默氏病研究中心 （ADRC）。神经病理学核心有助于获得尸检的大脑 经过评估的阿尔茨海默氏病（AD）病例的标本 然后是临床核心和AD患者的纵向 在犹太人的家和医院中确定的年龄很大 学术关联的疗养院。我们努力获得大脑 标本最短的验尸间隔和整个ADRC 标本收集39％可在4小时内获得69％的69％ 小时。样本以某种方式进行解剖和保存 最大化他们的实用性，满足了两个提出的实验的需求 在ADRC以及其他预期的研究项目中。这 包括快照冻结脑样本的一半并修复 另一半是新鲜准备的多聚甲醛。一个新的 解剖协议是允许准备的方法 大脑的选定区域以无偏见的采样方式 可以应用立体学的技术来量化病变和神经元 数字。进行了详细的神经病理学检查，以建立一个 神经病理学诊断以及记录的程度和 相关神经病变病变的分布。输入这些数据 进入广泛的数据库，可以集成到临床中 目的的数据库 调查。神经病理学核心还将提供帮助和 新开发的转基因的形态评估的专业知识 动物并解释任何已确定病变的性质 与AD或其他人类神经退行性条件的模型相关。 因为ADRC脑库已经运行了大约13 多年，是大脑银行的有效工作结构 已经存在。我们收集的组织已被广泛使用 在广泛的研究中。他们被广泛要求 ADRC中的研究人员，大西奈山研究社区 以及许多其他调查人员通过美国甚至国际上的调查员。 该核心的总体目的是继续维护和操作 大脑银行以满足核心和研究需求的方式 以最佳方式以ADRC进行。