CORE--ANTIOXIDANT ENZYME

核心--抗氧化酶

• 批准号: 6616896
• 负责人: LARRY OBERLEY
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-24 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6616896
• 关键词: antisense nucleic acid; biomedical facility; catalase; cell line; complementary DNA; enzyme activity; glutathione peroxidase; myeloperoxidase; superoxide dismutase; transfection /expression vector

DESCRIPTION (provided by applicant): The purpose of the Antioxidant Enzyme Core (AEC) is to provide antioxidant enzyme expertise and analysis for the individual research projects. Discussion between a project investigator and the AEC Director will be held to determine which experiments and/or assays are run. The AEC staff will carry out the proposed work or will advise the investigator on how to perform the experiments. Some of this work, particularly transfections of new cDNAs or in new cell lines as well as the development of antisense reagents, will be developmental in nature. The AEC will aid in enzyme activity assays, western, northern, and Southern analysis. The enzyme assays will be run on the antioxidant enzymes copper-and zinc-containing superoxide dismutase (CuZnSOD), manganese-containing superoxide dismutase (MnSOD), catalase (CAT), and glutathione peroxidase (GPx, both cytosolic and phospholipid). For Project 1, work will also be done on the white cell enzyme myeloperoxidase (MPO). For reagents, we have antibodies and cDNA probes to all primary antioxidant enzymes. In addition, the AEC will aid in transfecting the cDNAs for these enzymes into cell lines of interest to the project investigators. We have experience with several methods of transfection, as well as many different expression vectors for these enzymes. In the course of this work, the AEC will continue to maintain and develop reagents i.e., antibodies, cDNAs, and expression vectors for the antioxidant proteins. The AEC will also have as a goal the development of antisense reagents to inhibit the levels of all of the major antioxidant proteins.

描述（由申请人提供）：抗氧化酶的用途 核心（AEC）是为抗氧化酶提供专业知识和分析 个人研究项目。 项目研究者和项目研究者之间的讨论 AEC 主任将决定哪些实验和/或测定是 跑步。 AEC 工作人员将开展拟议的工作或向 研究者如何进行实验。 其中一些作品， 特别是新 cDNA 或新细胞系的转染以及 反义试剂的开发，本质上将是开发性的。 AEC 将协助西方、北方和南方的酶活性测定 分析。 酶测定将针对抗氧化酶铜和 含锌超氧化物歧化酶（CuZnSOD），含锰 超氧化物歧化酶 (MnSOD)、过氧化氢酶 (CAT) 和谷胱甘肽过氧化物酶 (GPx) 细胞质和磷脂）。 对于项目 1，还将完成以下工作： 白细胞髓过氧化物酶（MPO）。 对于试剂，我们有抗体 以及所有主要抗氧化酶的 cDNA 探针。 此外，AEC 将 帮助将这些酶的 cDNA 转染到感兴趣的细胞系中 项目调查员。 我们有多种方法的经验 转染，以及这些酶的许多不同的表达载体。 在这项工作的过程中，AEC 将继续维护和发展 试剂，即抗氧化剂的抗体、cDNA 和表达载体 蛋白质。 AEC 还将开发反义作为目标 抑制所有主要抗氧化蛋白水平的试剂。