CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

基本信息

批准号：
6344761
负责人：
PETER S RABINOVITCH
金额：
$ 10.06万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-06-01 至 2001-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6344761
关键词：
analytical chemistry biomedical equipment resource biomedical facility catalase flow cytometry glutathione glutathione peroxidase glutathione reductase glutathione transferase high performance liquid chromatography spectrometry superoxide dismutase

项目摘要

The Specific Aim of this Core is to provide two categories of services to the Program Project: 1) flow cytometry and cell sorting methods and technologies, and 2) assays of glutathione and the activity of redox enzymes glutathione-s-transferase, superoxide dismutase (S.O.D.) and catalase. We will also assist investigators in finding the most appropriate ways to apply these techniques to their research objectives and to interpret the experimental results. Cytometry and cell sorting will be essential parts of each of the program projects, providing variety of analytical services and techniques. Assays of DNA content, cell cycle and cell proliferation, a novel cytometric assay of oxidative DNA damage and repair, and cytometric assays related to resistance to oxidative damage (glutathione content, cell viability and apoptosis) will constitute major uses of cytometry in this Program Project proposal. In addition, this core will provide non-cytometric assays that will be widely used in the Program Project: HPLC and spectrophotometric quantitative assays of the important intracellular antioxidant glutathione; assays of glutathione-s- transferase, a principal enzyme responsible for carcinogen detoxification; assays of GSH peroxidase and GSH reductase, important enzymes in the synthesis of glutathione, and assays of the oxygen radial scavenging enzymes superoxide dismutase (S.O.D.) and catalase.

该核心的具体目标是提供两类服务该计划项目：1）流式细胞术和细胞分选方法以及技术，以及 2) 谷胱甘肽和氧化还原活性的测定谷胱甘肽-S-转移酶、超氧化物歧化酶 (S.O.D.) 和过氧化氢酶。我们还将协助调查人员找到最将这些技术应用于其研究目标的适当方法并解释实验结果。细胞计数和细胞分选将是每个计划项目的重要组成部分，提供各种分析服务和技术。 DNA含量、细胞周期和细胞增殖，氧化 DNA 损伤的新型细胞计数测定法与抗氧化损伤相关的修复和细胞计数测定（谷胱甘肽含量、细胞活力和细胞凋亡）将构成主要细胞计数术在本计划项目提案中的应用。另外，这个核心将提供将在该计划中广泛使用的非细胞计数检测项目：重要物质的 HPLC 和分光光度定量分析细胞内抗氧化剂谷胱甘肽；谷胱甘肽-s-的测定转移酶，负责致癌物解毒的主要酶； GSH 过氧化物酶和 GSH 还原酶（生物体内重要的酶）的测定谷胱甘肽的合成以及氧径向清除的测定酶超氧化物歧化酶（S.O.D.）和过氧化氢酶。