CORE--TRANSGENIC MOUSE PRODUCTION AND PATHOLOGY

核心——转基因小鼠的产生和病理学

• 批准号: 6345879
• 负责人: DENNIS LIGGITT
• 金额: $ 15.89万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345879
• 关键词: aging; animal breeding; animal colony; biomedical facility; catalase; data collection methodology /evaluation; genetically modified animals; laboratory mouse; neuropsychology; pathology; superoxide dismutase; transfection

Core C is essential component of the Gene Action Program Project which has always placed a high priority on analysis of aging effects on animal model systems. This tradition continues with a restructured emphasis oriented toward transgenic mice which overexpress genes which may influence the accumulative and composite effects of oxidative injury to nuclear and mitochondrial DNA. Core C will accomplish two major goals which will support each of the primary projects. The first goal is to provide transgenic and control mice which are maintained in a rigorous manner necessary to limit the influence of complicating factors such as intercurrent disease and nutrition on the aging process. This is supported by the first specific aim which is to provide breeding management and husbandry conditions necessary for the propagation of healthy and well- defined transgenic and control animals required by the projects and cores. The second goal is to provide an in-life and post-mortem analysis of transgenic and control mice throughout their natural lifespans. The second and third specific aims support this goal by (Aim 2) assessing the neurobehavioral influences of aging in transgenic and control mice during their natural lifespan, and (Aim 3) by establishing a reproductive and life table analysis and assessing the pathologic consequence of aging in transgenic and control mice during their natural lifespan. Accomplishment of these goals will permit associations to be developed between experimental observations made within individual projects and their ultimate expression in a integrated whole animal system.

核心 C 是基因行动计划项目的重要组成部分， 始终高度重视分析衰老对动物模型的影响 系统。 这一传统以重组的重点为导向继续延续 过度表达可能影响基因的转基因小鼠 氧化损伤对核和细胞的累积和复合效应 线粒体DNA。 Core C 将实现两个主要目标 支持每个主要项目。第一个目标是提供 以严格方式饲养的转基因小鼠和对照小鼠 有必要限制复杂因素的影响，例如 并发疾病和营养对衰老过程的影响。这是支持的 第一个具体目标是提供育种管理和 健康良好繁殖所必需的饲养条件 明确项目和核心所需的转基因和对照动物。 第二个目标是提供生前和事后分析 转基因小鼠和对照小鼠的整个自然寿命。第二个 第三个具体目标通过（目标 2）评估 衰老对转基因小鼠和对照小鼠神经行为的影响 它们的自然寿命，以及（目标 3）通过建立生殖和 生命表分析和评估衰老的病理后果 转基因小鼠和对照小鼠在其自然寿命期间。成就 这些目标将允许在之间建立联系 在各个项目中进行的实验观察及其 最终表达在一个完整的动物系统中。