Core--Vector

核心--向量

• 批准号: 6809071
• 负责人: ROBERT M. SAPOLSKY
• 金额: $ 20.94万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6809071
• 关键词: Adenoviridae; Alphaherpesvirinae; BCL2 gene /protein; Retroviridae; antioxidants; biomedical facility; biotechnology; catalase; gene expression; glutathione peroxidase; reporter genes; superoxide dismutase; transfection /expression vector

The vector core will produce herpes simplex virus (HSV)-based amplicon and retroviral vectors to be used in Projects by Giffard and Steinberg. Vectors will be produced expressing the genes for CuZn-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), combinations of the antioxidant genes, p35 and crmA and bcl-2. All the vectors will be "bicistronic," expressing both the gene under investigation and a reporter gene. For each experimental vector, the cognate control vector will contain the gene in question with a stop codon inserted in its center, insuring non-expression. Retroviral vectors to express SOD1, SOD2, catalase, GPx and combinations of the antioxidant genes will be produced. Control vectors will express either the reporter gene beta-galactosidase or a stop codon version of the gene of interest. The continuing purposes of the Vector Core are to ensure a constant supply of vectors for the research groups, insure quality control in the production of the vectors, and to troubleshoot problems encountered in the use of the vectors.

该载体核心将产生基于单纯疱疹病毒（HSV）的扩增子和逆转录病毒载体，用于吉法德和斯坦伯格的项目。 将产生表达铜锌超氧化物歧化酶 (SOD1)、锰 SOD (SOD2)、谷胱甘肽过氧化物酶 (GPX)、抗氧化剂基因组合、p35 和 crmA 以及 bcl-2 的基因的载体。 所有载体都将是“双顺反子”，既表达所研究的基因又表达报告基因。 对于每个实验载体，同源对照载体将包含所讨论的基因，并在其中心插入终止密码子，以确保不表达。 将产生表达 SOD1、SOD2、过氧化氢酶、GPx 和抗氧化基因组合的逆转录病毒载体。 对照载体将表达报告基因β-半乳糖苷酶或目的基因的终止密码子版本。 Vector Core的持续目标是确保为研究小组提供稳定的载体，确保载体生产中的质量控制，并解决载体使用中遇到的问题。