Ocular fluorophotometer for use with small eyes

适用于小眼睛的眼部荧光光度计

基本信息

批准号：
6994256
负责人：
Bruce M Ishimoto
金额：
$ 11.25万
依托单位：
OCUMETRICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-15 至 2006-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6994256
关键词：
anterior chamber blood aqueous barrier corneal endothelium fluoresceins fluorimetry intraocular aqueous flow laboratory mouse membrane permeability perfusion restraint retina technology /technique development

项目摘要

DESCRIPTION (provided by applicant): The purpose of this SBIR project is to develop an ocular fluorophotometer for measurement in the mouse eye. Ocular fluorophotometry has proven to be a useful and versatile technique in eye research. Small animal models of numerous ocular diseases, particularly in genetically engineered mice, present a unique opportunity. We propose to develop a system for performing ocular fluorophotometer on the mouse. Over the course of the entire project (both Phase I and Phase II), we intend to develop and test instrumentation and methods for measuring blood retinal barrier permeability, blood aqueous barrier permeability, aqueous turnover, cornea endothelial permeability and cornea epithelial permeability. During Phase I, we will limit our efforts to the measurement of aqueous turnover, but as we will explain, this will lay the foundation for the other applications and adequately answer the question of feasibility for those applications. During Phase II, we will address the other applications as well as further refine methods designed during Phase I. One area developed in Phase I that will remain a focus during Phase II will be the animal restraint system. It is our ultimate goal to develop a system that will allow rapid screening of many subjects.

描述（由申请人提供）：此SBIR项目的目的是开发一个眼部荧光表仪以在小鼠眼中进行测量。事实证明，眼部荧光光度法是眼科研究中的一种有用且通用的技术。众多眼疾病的小动物模型，尤其是在基因工程小鼠中，带来了独特的机会。我们建议开发一个用于在小鼠上执行眼部荧光计的系统。在整个项目的整个过程中（I期和第二阶段），我们打算开发和测试仪器以及测量血液视网膜屏障渗透性，血液屏障通透性，水性更新，角膜内皮通透性和角膜上皮渗透性的方法。在第一阶段，我们将限制我们的努力来测量水流动，但是正如我们将要解释的那样，这将为其他应用程序奠定基础，并充分回答对这些应用程序的可行性问题。在II阶段期间，我们将解决其他应用。在第一阶段中设计的进一步的完善方法，在第一阶段中开发的一个区域将在II期期间保持重点是动物约束系统。我们的最终目标是开发一个可以快速筛选许多主题的系统。