System for Ocular Oxygen Measurements

眼氧测量系统

• 批准号: 8002257
• 负责人: Bruce M Ishimoto
• 金额: $ 51.27万
• 依托单位: OCUMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2012-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8002257
• 关键词: Affinity; Animals; Aqueous Humor; Area; Budgets; Calibration; Cavia; Characteristics; Chemicals; Clinical; Communities; Computer software; Contact Lenses; Development; Devices; Diagnostic; Disease; Dyes; Electronics; Energy Transfer; Eye; Eye Development; Eye Movements; Feedback; Fluorescence; Fluorescence Spectroscopy; Frequencies; Functional disorder; Funding; Fundus; Goals; Grant; Gray unit of radiation dose; Health; Human; Imaging Techniques; In Vitro; Investigation; Laboratory Animals; Lasers; Lead; Life; Light; Location; Market Research; Marketing; Measurement; Measures; Methods; Microscopy; Monitor; Mus; Nature; New York; Noise; Ocular Physiology; Operative Surgical Procedures; Oryctolagus cuniculus; Output; Oxygen; Pharmaceutical Preparations; Phase; Photons; Physiologic pulse; Population; Primates; Procedures; Proteins; Protocols documentation; Publishing; Rattus; Research; Research Personnel; Retina; Retinal; Role; Scanning; Signal Transduction; Site; Solutions; Solvents; Source; Standardization; Sum; System; Techniques; Testing; Therapeutic; Time; Tissues; Work; base; biological systems; cost; design; detector; flexibility; fluorophore; human subject; improved; instrument; interest; meetings; operation; phosphorescence; prototype; sensor; theories; triplet state; usability; user-friendly

DESCRIPTION (provided by applicant): The purpose of this project is to develop systems for measuring oxygen concentrations on and inside the eye. These systems will take advantage of phosphorescent probes that are sensitive to oxygen concentrations. They will have obvious applications in eye research and will lead to advancements in our understanding of ocular diseases, development of new drugs, contact lenses and surgical procedures. It should be emphasized that these instrument systems are aimed solely at the ophthalmic research market, and NOT at the clinical diagnostic market. We believe that there is both a significant demand for such products in this market, and that their use will have significant impact to our understanding of oxygen in the eye, its role in ocular disease, and the development of new drugs, contact lenses, other therapeutic devices, and surgical procedures. Our initial technique used a dual frequency photon counting system, which we developed specifically for this purpose. It combined maximum sensitivity with the ability to measure a wide range of oxygen concentrations. During Phase I, we demonstrated feasibility of our techniques both in vitro and in the rabbit eye. These studies indicated that we could use a more flexible programmable photon counting system that could span a wide range of frequencies allowing the use of a wide range of phosphorescent probes. Since the last revision of this application, we have implemented this new design and tested it with both in vitro solutions and with live rabbits. There are a wide range of phosphorescent dyes available, and we propose to characterize many of them for their ability to penetrate or not penetrate into different ocular tissue. Phosphorescence quenching is not limited to oxygen. The dye system used in Phase I has previously been shown to be relatively insensitive to other ocular variables, but any new dyes will also have to be characterized similarly. A number of animal systems including humans have been used in previous research on ocular oxygen. We intend to leverage three delivery platforms developed by OcuMetrics to access the full range of animal systems: humans, laboratory animals (primates, rabbits, etc.), and very small laboratory animals (mice). Our overall goal is to develop multiple measurement systems based on dyes that have been selected for specific environmental affinity, oxygen range, etc. A critical goal will be standardization and calibration control, so that results made on different machines will be comparable. In addition, we will design this instrument with enough flexibility so that we can add capabilities as new phosphorescent oxygen probes and techniques are developed. PUBLIC HEALTH RELEVANCE: Oxygen is fundamental to life, but its presence is not only life giving, its reactive nature can also lead to dysfunction. The proposed instrument will take advantage of phosphorescent probes that are sensitive to oxygen concentrations to monitor oxygen concentrations in various areas of the eye. This will have obvious applications in eye research, and drug and contact lens testing.

描述（由申请人提供）：该项目的目的是开发用于测量眼睛内外氧气浓度的系统。这些系统将利用对氧浓度敏感的磷光探针。它们将在眼睛研究中明显应用，并将导致我们对眼部疾病，新药，隐形眼镜和手术程序的理解的进步。应该强调的是，这些仪器系统仅针对眼科研究市场，而不是针对临床诊断市场。我们认为，在这个市场上对这种产品既有需求，并且它们的使用将对我们对眼睛中的氧气，其在眼部疾病中的作用以及新药，隐形眼镜，其他治疗装置和外科手术的开发产生重大影响。我们的初始技术使用了双重频率光子计数系统，我们专门为此目的开发了该系统。它结合了最大灵敏度与测量广泛的氧气浓度的能力。在第一阶段，我们在体外和兔子眼中都证明了技术的可行性。这些研究表明，我们可以使用更灵活的可编程光子计数系统，该系统可能跨越广泛的频率，允许使用广泛的磷光探针。自从该应用程序的最后一次修订以来，我们已经实施了这一新设计，并使用体外解决方案和活兔子进行了测试。可提供广泛的磷光染料，我们建议其中许多染料的能力渗透或不穿透不同的眼组织。磷光淬灭不限于氧。先前已证明在第一阶段使用的染料系统对其他眼变量相对不敏感，但是任何新染料也必须类似地表征。先前关于眼氧的研究，包括人类在内的许多动物系统已被使用。我们打算利用Ocumetrics开发的三个输送平台来访问各种动物系统：人类，实验室动物（灵长类动物，兔子等）和非常小的实验室动物（小鼠）。我们的总体目标是开发基于针对特定环境亲和力，氧气范围等选择的染料的多个测量系统。关键目标将是标准化和校准控制，以便在不同机器上产生的结果将是可比的。此外，我们将以足够的灵活性设计该仪器，以便随着新的磷酸氧探针和技术的开发，我们可以添加功能。 公共卫生相关性：氧气是生命的基础，但其存在不仅是生命的奉献，其反应性质也会导致功能障碍。提出的仪器将利用对氧浓度敏感的磷光探针，以监测眼睛各个区域的氧气浓度。这将在眼睛研究，药物和隐形眼镜测试中有明显的应用。