Ocular fluorophotometer for use with small eyes

适用于小眼睛的眼部荧光光度计

• 批准号: 7159638
• 负责人: Bruce M Ishimoto
• 金额: $ 58.61万
• 依托单位: OCUMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7159638
• 关键词: cornea; eye; human; measurement

DESCRIPTION (provided by applicant): The purpose of this SBIR project is to develop an ocular fluorophotometer for measurements in the mouse eye. Ocular fluorophotometry has proven to be a useful and versatile technique in eye research. Small animal models of numerous ocular diseases, particularly in genetically engineered mice, present a unique opportunity to generate a large number of disease-specific results quickly that may be applicable to human eye research. This project proposes to develop a system for performing ocular fluorophotometry on the mouse. Over the course of both Phase I and Phase II, the goal is to develop and test both instrumentation and methods for measuring tear turnover, aqueous turnover, blood retinal barrier permeability, blood aqueous barrier permeability, cornea endothelial permeability, and cornea epithelial permeability. Phase I concentrated on the measurement of aqueous turnover. All Specific Aims were successfully attained with the demonstration of highly accurate instrumentation and appropriate methodology. This lays the foundation for implementing the other applications, all of which have been successfully measured in humans with instrumentation developed by this project s manufacturer. Phase II will address these other applications as well as further refine methods designed during Phase I. In particular, an animal restraint system will be designed in which each module holding a mouse can be quickly switched in and out of the main instrument. The ultimate goal is to measure first one eye, then the other eye, and then the eyes of the next mouse, all in rapid succession and without realignment of the instrument, so that many subjects can be screened in one measurement session. The overall result of these efforts will be to produce standardized instrumentation and methodology for eye research that can be used on mice for measuring multiple ophthalmic parameters in a standardized, repeatable, and comparable manner. Project Relevance Small animal models of numerous ocular diseases, particularly in genetically engineered mice, present a unique opportunity to generate a large number of disease-specific results quickly that may be applicable to human eye research. The proposed instrument will be used in conjunction with these animal models to advance our basic understanding of human eye disease and also to rapidly screen promising drugs and other disease therapies.

描述（由申请人提供）：该SBIR项目的目的是开发一个眼部荧光计，以在小鼠眼中进行测量。事实证明，眼部荧光光度法是眼科研究中的一种有用且通用的技术。众多眼疾病的小动物模型，特别是在基因工程小鼠中，提供了一个独特的机会，可以迅速产生大量疾病特异性结果，可能适用于人类眼睛的研究。该项目建议开发一种用于在小鼠上执行眼部荧光测量法的系统。在I阶段和II期的整个过程中，目标是开发和测试仪器和测量撕裂更新，水位更新，血液视网膜屏障通透性，血液水性屏障通透性，角膜内皮通透性和角膜上皮通透性的方法。第一阶段集中于水流量的测量。通过表现出高度准确的仪器和适当的方法，所有具体目标都成功实现了。这奠定了实施其他应用程序的基础，所有这些应用程序都是在该项目制造商开发的仪器中成功衡量的。第二阶段将解决这些其他应用程序，以及在第1阶段设计的进一步的完善方法，特别是，将设计一个动物约束系统，在该系统中，每个持有鼠标的模块都可以快速切换进出主仪器。最终的目标是测量第一只眼，然后是另一只眼睛，然后是下一只鼠标的眼睛，所有眼睛都是快速连续的，而无需仪器的重新调整，以便在一个测量过程中可以筛选许多受试者。这些努力的总体结果将是生产标准化的仪器和方法，用于眼睛研究，可用于小鼠以标准化，可重复和可比的方式测量多个眼科参数。项目相关性的许多眼部疾病的小动物模型，尤其是在基因工程的小鼠中，提供了一个独特的机会，可以迅速产生大量疾病特异性结果，可能适用于人眼研究。该拟议的仪器将与这些动物模型一起使用，以促进我们对人眼病的基本理解，并迅速筛查有希望的药物和其他疾病疗法。