Coordinated bioengineering of tooth and bone in the jaw

颌骨牙齿和骨骼的协调生物工程

• 批准号: 6954213
• 负责人: PAMELA C YELICK
• 金额: $ 18.87万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-28 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6954213
• 关键词: bioengineering /biomedical engineering; biotechnology; bone morphogenetic proteins; cell population study; dental pulp; dentin; epithelium; in situ hybridization; jaw; laboratory rat; ligaments; mesenchyme; oral pharyngeal surgery; periodontium; protein quantitation /detection; restorative dentistry; swine; tissue engineering; tissue support frame; tooth; tooth enamel; tooth root

DESCRIPTION (provided by applicant): Tooth loss continues to plague many Americans, and is complicated by bone resorption in the jaw. Current therapies for tooth replacement include synthetic dental implants, but these devices do not maintain the health of surrounding tissues and require sufficient amounts of alveolar bone to secure the implant. The ability to replace lost or diseased dentition and alveolar bone with healthy, biological teeth would revolutionize the practice of dentistry and oral surgery. Our long-term goal is to bioengineer biological tooth replacements containing enamel, dentin and pulp, and roots anchored to the underlying alveolar bone via periodontal ligament. Our current bioengineered tooth Crowns exhibit variable amounts of enamel and Jentin, and lack mature root structures. We expect that bioengineered tooth root formation will be enhanced by growth in close proximity to developing bone. This hypothesis will be tested by growing composite bioengineered tooth/bone constructs in the jaws of Yucatan mini-pigs. Excised implants will be analyzed for the presence of tooth crowns, mature tooth root structures, and alveolar bone. We will empirically determine the specific ratio of dental epithelial to mesenchymal cells seeded onto scaffolds required to bioengineer dentin and enamel of appropriate relative thickness. Thus, our Specific Aims are to: 1) demonstrate the coordinated formation of tooth crowns, roots and bone by bioengineering tooth and alveolar bone segments grown in the jaws of pigs, and 2) to bioengineer tooth crowns containing the relative amounts of enamel and dentin present in naturally formed teeth. We anticipate that the successful bioengineering of mature teeth anchored to alveolar bone via roots and periodontal ligament will provide an alternative therapeutic treatment for dental and oral cancer patients, and for individuals suffering from a variety of craniofacial defects.

描述（由申请人提供）： 牙齿脱落继续困扰着许多美国人，并且下颌的骨吸收使其复杂化。当前的牙齿替代疗法包括合成牙齿植入物，但是这些设备不能保持周围组织的健康状况，并且需要足够量的肺泡骨以固定植入物。用健康的生物牙齿替代丢失或患病的牙齿和肺泡骨的能力将彻底改变牙科和口腔手术的实践。我们的长期目标是含有牙釉质，牙本质和果肉的生物工具替代生物牙齿，并通过牙周韧带锚定在底部肺泡骨上的根。我们当前的生物工程牙冠表现出可变量的搪瓷和珍丁，并且缺乏成熟的根结构。我们预计，生物工程的牙根形成将通过与发育骨骼的近距离生长来增强。该假设将通过在Yucatan迷你杆的颌骨中种植复合生物工程的牙齿/骨构建体来检验。将分析切除的植入物，以便存在牙冠，成熟的牙根结构和牙槽骨。我们将凭经验确定牙齿上皮与生物工程牙本质所需的脚手架和适当相对厚度的搪瓷所需的间质细胞的特异性比率。因此，我们的具体目的是：1）通过生物工程牙齿和牙齿的牙齿骨骼片段在猪的颌骨中生长的牙齿和骨骼片段，以及2）牙齿牙齿的生物工程牙冠，这些牙齿和牙齿的相对量相对牙齿的牙齿与天然形成的牙齿相对量。我们预计，成熟牙齿的成功生物工程通过根和牙周韧带锚定在牙槽骨上，将为牙科和口腔癌患者以及患有多种颅面缺陷的人提供另一种治疗治疗。