Bioengineered Composite Alveolar Bone-Tooth Constructs for Tooth Regeneration

用于牙齿再生的生物工程复合牙槽骨牙齿结构

• 批准号: 9444207
• 负责人: PAMELA C YELICK
• 金额: $ 77.86万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9444207
• 关键词: Accidents; Address; Adult; Affect; Age; Alveolar; Alveolar Bone Loss; Animal Model; Athletic Injuries; Autologous; Biomedical Engineering; Biomimetics; Blood; Bone Regeneration; Bone Resorption; Bone Tissue; Cells; Child; Clinical Trials; Complex; Congenital Abnormality; Craniofacial Abnormalities; Data; Defect; Dental; Dental Implants; Dentistry; Dentition; Development; Dimensions; Disease; Excision; Exhibits; Extracellular Matrix; Family suidae; Gelatin; Genetic; Goals; Growth Factor; Harvest; Health; Health Priorities; Human; Hydrogels; Immune system; Implant; Individual; Injury; Interdisciplinary Study; Jaw; Knowledge; Live Birth; Malignant Neoplasms; Mandible; Maxilla; Methacrylates; Methods; Military Personnel; Minerals; Mission; Modeling; Morbidity - disease rate; Natural regeneration; Nerve; Operative Surgical Procedures; Oryctolagus cuniculus; Persons; Population; Property; Public Health; Publishing; Quality of life; Regenerative Medicine; Replacement Therapy; Reporting; Research; Signal Pathway; Silk; Site; Source; Techniques; Technology; Time; Tissue Engineering; Tissues; Tooth Germ; Tooth Loss; Tooth Tissue; Tooth regeneration; Tooth structure; United States; United States National Institutes of Health; aging population; alveolar bone; base; bioprinting; bone; cleft lip and palate; clinically relevant; craniofacial; craniofacial complex; craniofacial development; craniofacial repair; design; healing; improved; innovation; malignant mouth neoplasm; novel; novel strategies; oral surgery specialty; regenerative; repaired; scaffold; skeletal; tissue support frame

Project Summary/Abstract The coordinated development of craniofacial jaw bones and teeth are the result of a complex interplay between a surprisingly large and growing number of tissues - including blood and vasculature, and nerves – combined with the immune system, and a multitude of growth factor signaling pathways. Although knowledge of signaling pathways resulting in human mineralized tissue development has improved over the past few decades, detailed knowledge and understanding of how to regenerate human mineralized tissues in a functional and timely manner remains elusive – particularly with respect to the craniofacial complex and teeth. Here we propose novel strategies to effectively and coordinately regenerate alveolar jaw bone and tooth tissues, using three dimensional tissue engineering strategies. The objectives of the proposed studies, which build on our prior published reports, are to characterize two potential models for biomimetic three dimensional alveolar bone-tooth constructs: 1) natural decellularized tooth bud extracellular matrix scaffolds; and 2) Gelatin Methacrylate (GelMA) hydrogel scaffolds. Our published expertise in in the field of regenerative dentistry, mineralized tissue development and disease, strong preliminary data, and team of developmental biologists, clinicians and bioengineers supports our ability to accomplish the proposed Aims. We anticipate that the completion of the proposed studies will result in significantly improved knowledge and understanding of new methods to repair craniofacial defects caused by a variety of insults. The significance of the proposed studies and relevance to public health includes the facts that skeletal and craniofacial defects occur in as many as 1 in 700 live births in the United States alone, and that over a quarter of a million Maxillofacial surgeries were performed in 2014, including oral cancer resections. In addition, injuries to the craniofacial complex that include teeth are increasingly common due to accidents and sports injuries, and particularly in the battlefield where they represent up to 20% of injuries. As such, the proposed studies will make a significant contribution towards improved quality of life of both civilian and military populations.

项目摘要/摘要 颅面下颌骨头和牙齿的协调发展是由于复杂相互作用的结果 一个令人惊讶的大量组织，包括血液和脉管系统以及神经 - 合并 使用免疫系统和许多生长因子信号通路。尽管有信号的知识 在过去的几十年中，导致人类矿化组织发育的途径有所改善，详细 知识和理解如何在功能和及时的功能中再生人矿化组织 方式仍然难以捉摸 - 特别是在颅面复合物和牙齿方面。 在这里，我们提出了新的策略，以有效，协调再生肺泡颌骨和牙齿组织， 使用三维组织工程策略。拟议研究的对象，基于 我们先前发表的报告是为了表征两个仿生三维肺泡的潜在模型 骨齿构建体：1）天然脱细胞的牙芽细胞外基质支架； 2）明胶 甲基丙烯酸酯（Gelma）水凝胶支架。 我们在再生牙科，矿化组织发育和疾病领域发表的专业知识， 强大的初步数据，以及开发生物学家，临床医生和生物工程的团队支持我们的能力 实现拟议的目标。我们预计拟议的研究的完成将导致 显着提高了对修复颅面缺陷的新方法的了解和理解 各种侮辱。 拟议的研究的意义以及与公共卫生相关的重要性包括骨骼和 仅在美国，颅面缺陷就出现在700个活产中的多达1个，而四分之一以上 2014年，包括口腔癌切除术，进行了一百万个上颌面手术。另外，受伤 对于包括牙齿在内的颅面综合体而言，由于事故和运动损伤，越来越普遍 特别是在战场上，他们占受伤的20％。因此，拟议的研究将使 为改善平民和军事人口的生活质量做出了重大贡献。