Bioengineered Composite Alveolar Bone-Tooth Constructs for Tooth Regeneration

用于牙齿再生的生物工程复合牙槽骨牙齿结构

基本信息

批准号：
9444207
负责人：
PAMELA C YELICK
金额：
$ 77.86万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-15 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9444207
关键词：
Accidents Address Adult Affect Age Alveolar Alveolar Bone Loss Animal Model Athletic Injuries Autologous Biomedical Engineering Biomimetics Blood Bone Regeneration Bone Resorption Bone Tissue Cells Child Clinical Trials Complex Congenital Abnormality Craniofacial Abnormalities Data Defect Dental Dental Implants Dentistry Dentition Development Dimensions Disease Excision Exhibits Extracellular Matrix Family suidae Gelatin Genetic Goals Growth Factor Harvest Health Health Priorities Human Hydrogels Immune system Implant Individual Injury Interdisciplinary Study Jaw Knowledge Live Birth Malignant Neoplasms Mandible Maxilla Methacrylates Methods Military Personnel Minerals Mission Modeling Morbidity - disease rate Natural regeneration Nerve Operative Surgical Procedures Oryctolagus cuniculus Persons Population Property Public Health Publishing Quality of life Regenerative Medicine Replacement Therapy Reporting Research Signal Pathway Silk Site Source Techniques Technology Time Tissue Engineering Tissues Tooth Germ Tooth Loss Tooth Tissue Tooth regeneration Tooth structure United States United States National Institutes of Health aging population alveolar bone base bioprinting bone cleft lip and palate clinically relevant craniofacial craniofacial complex craniofacial development craniofacial repair design healing improved innovation malignant mouth neoplasm novel novel strategies oral surgery specialty regenerative repaired scaffold skeletal tissue support frame

项目摘要

Project Summary/Abstract The coordinated development of craniofacial jaw bones and teeth are the result of a complex interplay between a surprisingly large and growing number of tissues - including blood and vasculature, and nerves – combined with the immune system, and a multitude of growth factor signaling pathways. Although knowledge of signaling pathways resulting in human mineralized tissue development has improved over the past few decades, detailed knowledge and understanding of how to regenerate human mineralized tissues in a functional and timely manner remains elusive – particularly with respect to the craniofacial complex and teeth. Here we propose novel strategies to effectively and coordinately regenerate alveolar jaw bone and tooth tissues, using three dimensional tissue engineering strategies. The objectives of the proposed studies, which build on our prior published reports, are to characterize two potential models for biomimetic three dimensional alveolar bone-tooth constructs: 1) natural decellularized tooth bud extracellular matrix scaffolds; and 2) Gelatin Methacrylate (GelMA) hydrogel scaffolds. Our published expertise in in the field of regenerative dentistry, mineralized tissue development and disease, strong preliminary data, and team of developmental biologists, clinicians and bioengineers supports our ability to accomplish the proposed Aims. We anticipate that the completion of the proposed studies will result in significantly improved knowledge and understanding of new methods to repair craniofacial defects caused by a variety of insults. The significance of the proposed studies and relevance to public health includes the facts that skeletal and craniofacial defects occur in as many as 1 in 700 live births in the United States alone, and that over a quarter of a million Maxillofacial surgeries were performed in 2014, including oral cancer resections. In addition, injuries to the craniofacial complex that include teeth are increasingly common due to accidents and sports injuries, and particularly in the battlefield where they represent up to 20% of injuries. As such, the proposed studies will make a significant contribution towards improved quality of life of both civilian and military populations.

项目概要/摘要颅面颌骨和牙齿的协调发育是两者之间复杂相互作用的结果数量惊人且数量不断增加的组织（包括血液、脉管系统和神经）结合在一起与免疫系统和多种生长因子信号通路有关。由此产生的人类矿化组织发育途径在过去几十年中得到了改善，详细了解和理解如何及时、功能性地再生人体矿化组织方式仍然难以捉摸——特别是在颅面复合体和牙齿方面。在这里，我们提出了有效、协调地再生牙槽颌骨和牙齿组织的新策略，使用三维组织工程策略所提出的研究的目标是建立在这些策略的基础上的。我们之前发表的报告旨在描述仿生三维肺泡的两种潜在模型骨牙结构：1) 天然脱细胞牙芽细胞外基质支架；2) 明胶甲基丙烯酸酯（GelMA）水凝胶支架。我们在再生牙科、矿化组织发育和疾病领域发表的专业知识，强大的初步数据以及发育生物学家、主教和生物工程师团队支持我们的能力我们预计完成拟议的研究将导致：显着提高了对修复颅面部缺损新方法的认识和理解各种侮辱。拟议研究的重要性以及与公共卫生的相关性包括以下事实：骨骼和仅在美国，就有多达七百个活产儿中就有一个出现颅面缺陷，并且超过四分之一 2014 年进行了 100 万例颌面手术，其中包括口腔癌切除术以及损伤手术。由于事故和运动损伤，包括牙齿在内的颅面复合体越来越常见，并且特别是在战场上，它们所造成的伤害高达 20%，因此，拟议的研究将得出结论。对提高平民和军人的生活质量做出了重大贡献。