Core--Embryonic stem cell engineering

核心--胚胎干细胞工程

• 批准号: 7116676
• 负责人: ALEX L KOLODKIN
• 金额: $ 25.35万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116676
• 关键词: biomedical facility; biotechnology; embryonic stem cell; gene targeting; genetically modified animals; laboratory mouse; tissue /cell culture

The aim of the Embryonic Stem (ES) Cell Engineering facility is to accelerate research programs that depend on the development of genetically altered mouse models (knock-outs, knock-ins, and inducible knock-outs) that require ES cell manipulation. Successful ES cell engineering is a major hurdle for the realization of research goals for many NINDS investigators at JHU SOM (see below), and is currently performed by individual laboratories with varying degrees of success. The ES Cell Engineering Core will implement cutting-edge approaches for ES cell manipulation to assure that investigators use the most efficient strategies to generate animal models for NINDS supported research. These strategies will include generation of ES cells from existing mouse mutant lines for subsequent gene targeting and chimeric embryo production, and multi-hit transgenic targeting protocols that use novel recombination approaches for controlled site-directed mutagenesis. By establishing ES cell engineering as a core resource we will standardize protocols from the most successful laboratories, markedly increase the efficiency of model generation, and speed the pace of NINDS-supported research at JHU SOM. There is currently no core facility at JHU SOM that can perform ES cell engineering. The ES Cell Engineering Core will work in synergy with the current Transgenic Animal facility at JHU SOM, which provides complementary services such as injection of ES cells into blastocysts and animal husbandry necessary to achieve high chimera mice. There will be no overlap in the services provided by the ES Cell Engineering Core and the Transgenic Animal facility.

胚胎茎（ES）细胞工程设施的目的是加速依赖于需要ES细胞操作的遗传改变的小鼠模型（敲除，敲门和可诱导的敲除）的研究计划。成功的ES细胞工程是实现JHU SOM许多NINDS研究人员（见下文）研究目标的主要障碍，目前由单个实验室取得了不同程度的成功。 ES细胞工程核心将实施ES细胞操作的尖端方法，以确保研究人员使用最有效的策略来为NINDS支持的研究生成动物模型。这些策略将包括从现有小鼠突变系的ES生成，以进行后续基因靶向和嵌合胚产生以及多重打击 转基因靶向方案，使用新型重组方法来控制位置定向的诱变。通过将ES工程建立为核心资源，我们将标准化最成功的实验室的协议，显着提高了模型生成的效率，并加快了JHU SOM的NINDS支持研究的速度。 JHU SOM目前没有可以执行ES Cell工程的核心设施。 ES细胞工程核心将与Jhu SOM的当前转基因动物设施协同作用， 提供互补的服务，例如将ES细胞注射到胚泡和畜牧业中，以实现高嵌合体小鼠。 ES细胞工程核心和转基因动物设施提供的服务不会重叠。