CORE-- Imaging and Cytometry Facilities Core

CORE--成像和细胞计数设施核心

• 批准号: 6750900
• 负责人: Kimberley J Woodcroft
• 金额: $ 16.32万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6750900
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; environmental toxicology; flow cytometry; imaging /visualization /scanning; immunocytochemistry

Description (provided by applicant): The mission of the Imaging and Cytometry Facility Core (ICFC) is to support established, peer-reviewed, funded research programs of EHS Center members by providing access to contemporary technology, instrumentation, and services in microscopy and flow cytometry at little or no cost to individual investigators. The ICFC enhances the research productivity of Center members by providing the instrumentation, training, and expertise necessary to successfully adopt microscopic, flow cytometric, and imaging techniques into both their individual and collaborative research programs. During the past funding period, the 30 investigators utilizing ICFC services and facilities have published 83 manuscripts and received funding for 29 grants (new and renewal), with another 3 pending, utilizing data generated through ICFC use. Access to these technologies is essential for pursuing studies in environmental, cellular, and molecular toxicology, especially those in signal transduction. The ICFC has fostered multi-disciplinary collaborative research in the areas of xenobiotic effects on a variety of cell/organ systems, the effects of heavy metals on cell function and signalling, the regulation of xenobiotic metabolizing enzyme gene expression, DNA repair in response to toxic insult, regulation of cellular signalling pathways, cellular responses to oxidative stress, and T-cell phenotype association with asthma susceptibility. The ICFC are to attract new users and new Center members. Since the last competitive renewal, 16 new users, all Center members, have utilized the ICFC services/facilities. The ICFC facilitates the introduction of new approaches to investigators' individual and collaborative research programs through the provision of services requiring little initial input of time or money on the part of the investigator. The ICFC provides access to technologies and instrumentation that would otherwise be unavailable to individual investigators, and strives to introduce new technologies in response to the Center investigator needs. Advanced equipment is maintained and operated by expert core personnel, who provide service, guidance, and training to EHS Center members using the facility. Investigators can receive hands-on training in light, confocal, and fluorescent microscopy, immunocytochemistry, flow cytometry and cell sorting, and the comet assay. The ICFC also maintains equipment and instrumentation for sample analysis (e.g., spectrophotometers, fluorometer, luminometer, Agilent Bioanalyzer) and data acquisition (e.g., gel documentation system, film developer, scanning laser densitometer). The goal of the ICFC is to acquire and maintain these instruments and services in a functional multi-user environment normally unavailable to individual investigators.

描述（由申请人提供）：成像和细胞术设施核心（ICFC）的使命是为了支持建立，经过同行评审，资助的EHS中心成员的研究计划，通过提供当代技术，仪器和服务中的仪器和流式细胞仪的访问，以少量或无需为个人调查人员提供费用。 ICFC通过提供仪器，培训和专业知识来成功地采用显微镜，流式细胞术和成像技术，从而提高了中心成员的研究生产率。在过去的资金期间，使用ICFC服务和设施的30名调查人员出版了83份手稿，并获得了29个赠款（New and Renewal）的资金，另有3个待定，并利用了通过ICFC使用生成的数据。获取这些技术对于追求环境，细胞和分子毒理学，尤其是信号转导的研究至关重要。 ICFC培养了在该领域的多学科合作研究 异种生物对各种细胞/器官系统的影响，重金属对细胞功能和信号传导的影响，异种生物代谢的调节酶基因表达，对毒性损伤的DNA修复，细胞信号传导的调节，细胞信号途径的调节，细胞对氧化应激的响应以及氧化应激的响应以及T细胞现象型与Asthmamectipity的关联。 ICFC将吸引新用户和新中心成员。自上次竞争性续约以来，16个新用户，所有中心成员都使用了ICFC服务/设施。 ICFC促进了通过提供调查员几乎不需要时间或金钱的服务的服务来引入调查人员的个人和协作研究计划的新方法。 ICFC提供了对个人调查人员无法获得的技术和仪器的访问，并努力介绍新技术以应对中心研究人员的需求。高级设备由专家核心人员维护和运营，他们使用该设施为EHS中心成员提供服务，指导和培训。研究人员可以接受光，共聚焦和荧光显微镜，免疫细胞化学，流式细胞仪和细胞分选以及彗星测定法进行动手训练。 ICFC还维护了用于样品分析的设备和仪器（例如分光光度计计，荧光计，发光计，安捷伦生物分析仪）和数据采集（例如，凝胶文档系统，胶片开发人员，扫描激光密度计）。 ICFC的目标是在通常无法为个别调查人员提供的功能多用户环境中获取和维护这些工具和服务。