Immuno-Epidemiology Research Laboratory Core

免疫流行病学研究实验室核心

• 批准号: 8843150
• 负责人: Kimberley J Woodcroft
• 金额: $ 45.2万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8843150
• 关键词: Allergens; Applications Grants; Archives; Asthma; Biological; Biological Assay; Biometry; Blood; Blood specimen; Cell Culture Techniques; Cells; Collection; County; DNA; Data; Data Sources; Dendritic Cells; Dust; Endotoxins; Environment; Epidemiologic Studies; Equipment; Extrinsic asthma; Feces; Flow Cytometry; Future; Goals; Health; Health Sciences; Home environment; House Dust; Human Milk; Hypersensitivity; IgE; Immune; Immunoglobulins; Immunology procedure; Individual; Infant; Instruction; Laboratories; Learning; Longitudinal Studies; Manuscripts; Participant; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Process; Program Research Project Grants; Protocols documentation; Publications; RNA; RNA analysis; Regulatory T-Lymphocyte; Research Personnel; Resources; Sampling; Serum; Services; Shipping; Ships; Specimen; T-Cell Activation; Techniques; Technology; Time; Training; Universities; Whole Blood; biobank; cost; cytokine; data integration; data management; experience; microbial community; microbiome; pet animal; repository; research study; synthetic polymer Bioplex

PROJECT SUMMARY (See instructions): The purpose of the Immuno-Epidemiology Research Laboratory Core (Core C) is to provide a technical, intellectual and physical resource for the investigators in this Program Project Grant application. This laboratory has substantial experience performing all of the tasks and assays that are proposed for this Core in the present proposal and has been providing these services for the WHEALS project since its inception. As the central processing laboratory, Projects 1, 2, 3 and 4 will rely on Core C to process, track, store and/or ship archived and newly acquired blood, stool and dust specimens. Additionally, for Project 2, Core C will perform the isolation, culture and stimulation of peripheral blood mononuclear cells (PBMCs); dendritic cell and regulatory T cell phenotyping by multicolor flow cytometry; RNA analysis by realfime PCR; and Bio-Plex assay of secreted cytokines following stimulation of cultured PBMCs. The Specific Aims of Core C are: 1) To serve as the central collection, storage and processing laboratory for blood and dust samples collected in Projects 1, 2, 3 and 4; to ship plasma and dust samples to the dust and IgE Analysis Core (Core E) for Projects 2, 3, and 4; to ship stool samples to the Microbial Community Analysis Core (Core D) for Projects 1, 2 and 4; and, to ship processed dust samples collected in Project 2 to Dr. Lukacs' lab for use in Project 3, 2) To perform validated flow cytometric assays for phenotyping of dendritic cells and Tregs, and to perform validated Bio-Plex assays of secreted cytokines in stimulated PBMC culture supernatants for Project 2, in an efficient, reliable and consistent fashion, 3) To sen/e as a biorepository for plasma, DNA, RNA, dust, stool and other biological samples for use in future studies, 4) To provide data obtained from flow cytometry and Bio-Plex analyses to the Biostatistics and Data Management Core (Core B) for integration with data from Cores D and E and other data sources for statistical analyses for manuscripts and additional grant applications, and 5) To collaborate with the other investigators in the interpretation and integration of immunologic assay results into publications.

项目摘要（请参阅说明）： 免疫 - 流行病学研究实验室核心（Core C）的目的是为调查人员在该计划项目赠款应用中提供技术，智力和物理资源。该实验室具有执行本提案中为此核心提出的所有任务和测定的实质性经验，并且自成立以来就一直为Wheals项目提供这些服务。 作为中央加工实验室，项目1、2、3和4将依靠Core C处理，跟踪，存储和/或船舶存档以及新获得的血液，凳子和灰尘标本。此外，对于项目2，核心C将 进行外周血单核细胞（PBMC）的分离，培养和刺激；多色流式细胞仪的树突状细胞和调节性T细胞表型； Realfime PCR的RNA分析；刺激培养的PBMC后，分泌细胞因子的生物质量测定。核心C的具体目的是：1）作为在项目1、2、3和4中收集的血液和灰尘样品的中央收集，存储和加工实验室；将等离子体和灰尘样品运送到灰尘和IgE分析核心（核心E） 项目2、3和4；将粪便样品运送到项目1、2和4的微生物社区分析核心（核心D）；并且，要在项目2中收集到卢卡克斯博士实验室的运输加工的灰尘样品，以在项目3，2）中使用，以执行经过验证的流式细胞术测定，用于对树突状细胞和Tregs的表型进行验证，并在刺激的PBMC培养超级段的刺激的PBMC培养超级段的分泌细胞因子进行验证的Bio-plex测定。 3）3）作为等离子体，DNA，RNA，粉尘，粪便，粪便和其他生物样品的生物座，用于未来的研究中，4）提供数据，以提供流式细胞仪和生物 - 密型分析，以与生物稳定和数据管理（核心核心）的批准和其他数据进行分析，并提供其他分析和其他数据的核心（核心），并提供了其他分析的数据，并提供了其他数据，并将其分析和其他数据列表和其他数据进行分析，并提供了其他分析的数据。应用程序，以及5）与其他研究人员合作，以解释和整合免疫学分析结果到出版物中。